PRIMER_SEQUENCE_ID=NM_172098
SEQUENCE=GGGTCACGCTAACGCCGCGGTTTCCTCCGCTCGATTGGTTCTACTGTGGGTCTGGACTGATCTCCATGTCCTGTTGTGGGGCTTTTACAGCCTTTGGATTGTGAAAACTGCTGAGAGAGACTTGCAATCCAGTCACATAAGTATAATAAAGAAATATTGGTCCTCATGGAAGAAGAGCAAGATTTACCAGAGCAACCAGTGAAAAAAGCCAAGATGCAGGAATCAGGAGAGCAAACTATAAGTCAAGTAAGCAATCCAGATGTCAGTGATCAGAAGCCTGAAACATCAAGCCTTGCTTCAAACCTTCCCATGTCAGAGGAAATTATGACATGCACCGATTACATCCCTCGCTCATCCAATGATTATACCTCACAAATGTATTCTGCAAAACCTTATGCACATATTCTCTCAGTTCCTGTTTCGGAAACTGCTTACCCTGGACAGACTCAATACCAGACACTACAGCAGACTCAACCCTATGCTGTCTACCCTCAGGCAACCCAAACGTATGGACTACCTCCTTTTGGTGCATTGTGGCCAGGTATGAAACCTGAAAGTGGTTTAATTCAGACTCCATCTCCAAGTCAACACAGTGTTCTTACCTGCACTACAGGGTTAACCACAAGCCAGCCAAGCCCAGCACATTATTCTTATCCCATTCAAGCTTCAAGCACAAATGCCAGCCTGATATCTACTTCTTCTACAATTGCCAATATTCCAGCAGCAGCAGTAGCCAGCATCTCAAACCAGGATTATCCCACCTATACTATTCTTGGTCAGAATCAGTACCAGGCCTGCTACCCCAGCTCCAGCTTTGGAGTCACAGGTCAGACTAACAGTGATGCAGAGAGCACCACATTAGCAGCAACCACATACCAGTCGGAGAAGCCTAGTGTCATGGCGCCTGCACCTGCAGCACAGAGACTTTCCTCTGGAGACCCTTCTACAAGTCCATCTTTGTCCCAGACTACACCAAGTAAAGATACTGATGATCAGTCCAGGAAAAACATGACTAGCAAGAACCGGGGCAAGAGGAAAGCTGATGCCACTTCTTCCCAAGACAGTGAATTAGAACGGGTATTTCTGTGGGACTTGGATGAAACCATCATCATCTTCCACTCACTTCTTACTGGATCCTATGCCCAGAAATATGGAAAGGACCCAACAGTAGTGATTGGCTCAGGTTTAACAATGGAAGAAATGATTTTTGAAGTGGCTGATACTCATCTATTTTTCAATGACTTAGAGACATAGTCCTCACTCAGGAACTGAAAGTTCTGTGAAGAATTTGACCTGAATGTGTTCCTGCCCCCCAGGAGTGTGACCAGGTACATGTGGAAGATGTGGCTTCTGATGACAATGGCCAAGACTTGAGCAACTACAGTTTCTCAACAGATGGTTTCAGTGGCTCAGGAGGTAGTGGCAGCCATGGTTCATCTGTGGGTGTTCAGGGAGGTGTGGACTGGATGAGGAAACTAGCTTTCCGCTACCGGAAAGTGAGAGAAATCTATGATAAGCATAAAAGCAACGTGGGTGGTCTCCTCAGTCCCCAGAGGAAGGAAGCACTGCAGAGATTAAGAGCAGAAATTGAAGTTTTAACAGATTCCTGGTTAGGAACTGCATTAAAGTCCTTACTTCTCATCCAGTCCAGAAAGAATTGTGTGAATGTTCTGATCACTACCACCCAGCTGGTTCCAGCCCTGGCCAAGGTTCTCCTATATGGACTAGGAGAAATATTTCCTATTGAGAACATCTATAGTGCTACCAAAATTGGTATGGTTTCTATGCTGTTTCTTGTCACTGGCCTTGCAATAAAACTTTCTACTCATGATGCAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 20706, GC content failed 129, low tm 10865, high tm 4672, high any compl 4, high end compl 27, high repeat similarity 34, long poly-x seq 42, ok 4933
PRIMER_RIGHT_EXPLAIN=considered 20602, GC content failed 166, low tm 11099, high tm 4295, high any compl 2, high end compl 4, high repeat similarity 8, long poly-x seq 58, ok 4970
PRIMER_PAIR_EXPLAIN=considered 323, unacceptable product size 263, high end compl 8, ok 52
PRIMER_PAIR_PENALTY=0.0498
PRIMER_LEFT_PENALTY=0.040223
PRIMER_RIGHT_PENALTY=0.009608
PRIMER_LEFT_SEQUENCE=CTACAAGTCCATCTTTGTCCCAG
PRIMER_RIGHT_SEQUENCE=ACAATTCTTTCTGGACTGGATGA
PRIMER_LEFT=943,23
PRIMER_RIGHT=1660,23
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=24.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=718
PRIMER_PAIR_PENALTY_1=0.0779
PRIMER_LEFT_1_PENALTY=0.045432
PRIMER_RIGHT_1_PENALTY=0.032476
PRIMER_LEFT_1_SEQUENCE=CCTGGACAGACTCAATACCAGAC
PRIMER_RIGHT_1_SEQUENCE=ACTGGTATGTGGTTGCTGCTAAT
PRIMER_LEFT_1=435,23
PRIMER_RIGHT_1=879,23
PRIMER_LEFT_1_TM=60.045
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=445
PRIMER_PAIR_PENALTY_2=0.0809
PRIMER_LEFT_2_PENALTY=0.048411
PRIMER_RIGHT_2_PENALTY=0.032476
PRIMER_LEFT_2_SEQUENCE=ATTTACCAGAGCAACCAGTGAAA
PRIMER_RIGHT_2_SEQUENCE=ACTGGTATGTGGTTGCTGCTAAT
PRIMER_LEFT_2=181,23
PRIMER_RIGHT_2=879,23
PRIMER_LEFT_2_TM=60.048
PRIMER_RIGHT_2_TM=59.968
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=699
PRIMER_PAIR_PENALTY_3=0.0857
PRIMER_LEFT_3_PENALTY=0.045432
PRIMER_RIGHT_3_PENALTY=0.040223
PRIMER_LEFT_3_SEQUENCE=CCTGGACAGACTCAATACCAGAC
PRIMER_RIGHT_3_SEQUENCE=CTGGGACAAAGATGGACTTGTAG
PRIMER_LEFT_3=435,23
PRIMER_RIGHT_3=965,23
PRIMER_LEFT_3_TM=60.045
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=531
PRIMER_PAIR_PENALTY_4=0.0886
PRIMER_LEFT_4_PENALTY=0.048411
PRIMER_RIGHT_4_PENALTY=0.040223
PRIMER_LEFT_4_SEQUENCE=ATTTACCAGAGCAACCAGTGAAA
PRIMER_RIGHT_4_SEQUENCE=CTGGGACAAAGATGGACTTGTAG
PRIMER_LEFT_4=181,23
PRIMER_RIGHT_4=965,23
PRIMER_LEFT_4_TM=60.048
PRIMER_RIGHT_4_TM=60.040
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=785
PRIMER_PAIR_PENALTY_5=0.0951
PRIMER_LEFT_5_PENALTY=0.085505
PRIMER_RIGHT_5_PENALTY=0.009608
PRIMER_LEFT_5_SEQUENCE=ATCTTTGTCCCAGACTACACCAA
PRIMER_RIGHT_5_SEQUENCE=ACAATTCTTTCTGGACTGGATGA
PRIMER_LEFT_5=953,23
PRIMER_RIGHT_5=1660,23
PRIMER_LEFT_5_TM=59.914
PRIMER_RIGHT_5_TM=59.990
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=708
PRIMER_PAIR_PENALTY_6=0.0995
PRIMER_LEFT_6_PENALTY=0.067019
PRIMER_RIGHT_6_PENALTY=0.032476
PRIMER_LEFT_6_SEQUENCE=CTGAGAGAGACTTGCAATCCAGT
PRIMER_RIGHT_6_SEQUENCE=ACTGGTATGTGGTTGCTGCTAAT
PRIMER_LEFT_6=110,23
PRIMER_RIGHT_6=879,23
PRIMER_LEFT_6_TM=60.067
PRIMER_RIGHT_6_TM=59.968
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=5.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=770
PRIMER_PAIR_PENALTY_7=0.1072
PRIMER_LEFT_7_PENALTY=0.067019
PRIMER_RIGHT_7_PENALTY=0.040223
PRIMER_LEFT_7_SEQUENCE=CTGAGAGAGACTTGCAATCCAGT
PRIMER_RIGHT_7_SEQUENCE=CTGGGACAAAGATGGACTTGTAG
PRIMER_LEFT_7=110,23
PRIMER_RIGHT_7=965,23
PRIMER_LEFT_7_TM=60.067
PRIMER_RIGHT_7_TM=60.040
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=5.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=856
PRIMER_PAIR_PENALTY_8=0.1146
PRIMER_LEFT_8_PENALTY=0.040223
PRIMER_RIGHT_8_PENALTY=0.074349
PRIMER_LEFT_8_SEQUENCE=CTACAAGTCCATCTTTGTCCCAG
PRIMER_RIGHT_8_SEQUENCE=ATGATGATGGTTTCATCCAAGTC
PRIMER_LEFT_8=943,23
PRIMER_RIGHT_8=1111,23
PRIMER_LEFT_8_TM=60.040
PRIMER_RIGHT_8_TM=60.074
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=169
PRIMER_PAIR_PENALTY_9=0.1146
PRIMER_LEFT_9_PENALTY=0.040223
PRIMER_RIGHT_9_PENALTY=0.074349
PRIMER_LEFT_9_SEQUENCE=CTACAAGTCCATCTTTGTCCCAG
PRIMER_RIGHT_9_SEQUENCE=GTGGAAGATGATGATGGTTTCAT
PRIMER_LEFT_9=943,23
PRIMER_RIGHT_9=1118,23
PRIMER_LEFT_9_TM=60.040
PRIMER_RIGHT_9_TM=60.074
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=176
PRIMER_PAIR_PENALTY_10=0.1198
PRIMER_LEFT_10_PENALTY=0.045432
PRIMER_RIGHT_10_PENALTY=0.074349
PRIMER_LEFT_10_SEQUENCE=CCTGGACAGACTCAATACCAGAC
PRIMER_RIGHT_10_SEQUENCE=GTGGAAGATGATGATGGTTTCAT
PRIMER_LEFT_10=435,23
PRIMER_RIGHT_10=1118,23
PRIMER_LEFT_10_TM=60.045
PRIMER_RIGHT_10_TM=60.074
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=684
PRIMER_PAIR_PENALTY_11=0.1198
PRIMER_LEFT_11_PENALTY=0.045432
PRIMER_RIGHT_11_PENALTY=0.074349
PRIMER_LEFT_11_SEQUENCE=CCTGGACAGACTCAATACCAGAC
PRIMER_RIGHT_11_SEQUENCE=ATGATGATGGTTTCATCCAAGTC
PRIMER_LEFT_11=435,23
PRIMER_RIGHT_11=1111,23
PRIMER_LEFT_11_TM=60.045
PRIMER_RIGHT_11_TM=60.074
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=677
PRIMER_PAIR_PENALTY_12=0.1228
PRIMER_LEFT_12_PENALTY=0.048411
PRIMER_RIGHT_12_PENALTY=0.074349
PRIMER_LEFT_12_SEQUENCE=ATTTACCAGAGCAACCAGTGAAA
PRIMER_RIGHT_12_SEQUENCE=ATGATGATGGTTTCATCCAAGTC
PRIMER_LEFT_12=181,23
PRIMER_RIGHT_12=1111,23
PRIMER_LEFT_12_TM=60.048
PRIMER_RIGHT_12_TM=60.074
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=931
PRIMER_PAIR_PENALTY_13=0.1276
PRIMER_LEFT_13_PENALTY=0.045432
PRIMER_RIGHT_13_PENALTY=0.082173
PRIMER_LEFT_13_SEQUENCE=CCTGGACAGACTCAATACCAGAC
PRIMER_RIGHT_13_SEQUENCE=TTGAAGCTTGAATGGGATAAGAA
PRIMER_LEFT_13=435,23
PRIMER_RIGHT_13=669,23
PRIMER_LEFT_13_TM=60.045
PRIMER_RIGHT_13_TM=60.082
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=8.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=235
PRIMER_PAIR_PENALTY_14=0.1306
PRIMER_LEFT_14_PENALTY=0.048411
PRIMER_RIGHT_14_PENALTY=0.082173
PRIMER_LEFT_14_SEQUENCE=ATTTACCAGAGCAACCAGTGAAA
PRIMER_RIGHT_14_SEQUENCE=TTGAAGCTTGAATGGGATAAGAA
PRIMER_LEFT_14=181,23
PRIMER_RIGHT_14=669,23
PRIMER_LEFT_14_TM=60.048
PRIMER_RIGHT_14_TM=60.082
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=34.783
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=489
PRIMER_PAIR_PENALTY_15=0.1339
PRIMER_LEFT_15_PENALTY=0.048411
PRIMER_RIGHT_15_PENALTY=0.085505
PRIMER_LEFT_15_SEQUENCE=ATTTACCAGAGCAACCAGTGAAA
PRIMER_RIGHT_15_SEQUENCE=TTGGTGTAGTCTGGGACAAAGAT
PRIMER_LEFT_15=181,23
PRIMER_RIGHT_15=975,23
PRIMER_LEFT_15_TM=60.048
PRIMER_RIGHT_15_TM=59.914
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=6.6000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=795
PRIMER_PAIR_PENALTY_16=0.1478
PRIMER_LEFT_16_PENALTY=0.045432
PRIMER_RIGHT_16_PENALTY=0.102347
PRIMER_LEFT_16_SEQUENCE=CCTGGACAGACTCAATACCAGAC
PRIMER_RIGHT_16_SEQUENCE=ACCCTGTAGTGCAGGTAAGAACA
PRIMER_LEFT_16=435,23
PRIMER_RIGHT_16=615,23
PRIMER_LEFT_16_TM=60.045
PRIMER_RIGHT_16_TM=60.102
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=181
PRIMER_PAIR_PENALTY_17=0.1492
PRIMER_LEFT_17_PENALTY=0.067019
PRIMER_RIGHT_17_PENALTY=0.082173
PRIMER_LEFT_17_SEQUENCE=CTGAGAGAGACTTGCAATCCAGT
PRIMER_RIGHT_17_SEQUENCE=TTGAAGCTTGAATGGGATAAGAA
PRIMER_LEFT_17=110,23
PRIMER_RIGHT_17=669,23
PRIMER_LEFT_17_TM=60.067
PRIMER_RIGHT_17_TM=60.082
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=34.783
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=8.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=560
PRIMER_PAIR_PENALTY_18=0.1508
PRIMER_LEFT_18_PENALTY=0.048411
PRIMER_RIGHT_18_PENALTY=0.102347
PRIMER_LEFT_18_SEQUENCE=ATTTACCAGAGCAACCAGTGAAA
PRIMER_RIGHT_18_SEQUENCE=ACCCTGTAGTGCAGGTAAGAACA
PRIMER_LEFT_18=181,23
PRIMER_RIGHT_18=615,23
PRIMER_LEFT_18_TM=60.048
PRIMER_RIGHT_18_TM=60.102
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=7.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=435
PRIMER_PAIR_PENALTY_19=0.1525
PRIMER_LEFT_19_PENALTY=0.067019
PRIMER_RIGHT_19_PENALTY=0.085505
PRIMER_LEFT_19_SEQUENCE=CTGAGAGAGACTTGCAATCCAGT
PRIMER_RIGHT_19_SEQUENCE=TTGGTGTAGTCTGGGACAAAGAT
PRIMER_LEFT_19=110,23
PRIMER_RIGHT_19=975,23
PRIMER_LEFT_19_TM=60.067
PRIMER_RIGHT_19_TM=59.914
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=866
=