PRIMER_SEQUENCE_ID=NM_172112
SEQUENCE=GCAGAGCTCAACTCTGCCCCTCAAACCACAATAGGAACCAGTGGTTTCCGGGTGATCCTGATGACAGTTTCCAGTGTCTACCTGGAATGAAACGAATGGTACAAGGAAATGGTAGAACTAGTGATCTCACCCAGCCTCACTGTAAACAGCGATTGTCTGGATAAACTGAAGTTTAACCGTGCTGACGCTGCTGTGTGGACTCTGAGTGACAGACAAGGCATCACCAAATCGGCCCCCCTGAGAGTGTCCCAGCTCTTCTCCAGATCTTGCCCACGTGTCCTCCCCCGCCAGCCTTCCACAGCCATGGCAGCCTACGGCCAGACGCAGTACAGTGCGGGGATCCAGCAGGCTACCCCCTATACAGCTTACCCACCTCCAGCACAAGCCTATGGAATCCCTTCCTACAGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTGGGTTGATAGGCACTCCCAAAAGGGAGACCTGGCTACAGCTCCGAGCTGAGCTGGAAGCTCTCACAGACCTCTGGCTGACCCACTCCCTGAAGGCACTAAACCTCATCAACTCCCGGCCCAACTGTGTCAATGTGCTGGTCACCACCACTCAACTAATTCCTGCCCTGGCCAAAGTCCTGCTATATGGCCTGGGGTCTGTGTTTCCTATTGAGAACATCTACAGTGCAACCAAGACAGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGTGATGGATAAAAGGCATTAAATAAAACCACGTTTACATTTTGAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 27813, GC content failed 332, low tm 9891, high tm 11605, high any compl 6, high end compl 19, high repeat similarity 17, long poly-x seq 63, ok 5880
PRIMER_RIGHT_EXPLAIN=considered 27785, GC content failed 547, low tm 9860, high tm 11454, high any compl 3, high end compl 6, high repeat similarity 8, long poly-x seq 71, ok 5836
PRIMER_PAIR_EXPLAIN=considered 244, unacceptable product size 193, high end compl 6, ok 45
PRIMER_PAIR_PENALTY=0.0872
PRIMER_LEFT_PENALTY=0.046971
PRIMER_RIGHT_PENALTY=0.040223
PRIMER_LEFT_SEQUENCE=AGTGTCTACCTGGAATGAAACGA
PRIMER_RIGHT_SEQUENCE=CTCCTTGATAGAACCCTGTTGTG
PRIMER_LEFT=72,23
PRIMER_RIGHT=546,23
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=475
PRIMER_PAIR_PENALTY_1=0.1140
PRIMER_LEFT_1_PENALTY=0.085505
PRIMER_RIGHT_1_PENALTY=0.028502
PRIMER_LEFT_1_SEQUENCE=TCACCACCACTCAACTAATTCCT
PRIMER_RIGHT_1_SEQUENCE=CCTTGCTTTTGTTCTGTGAGTCT
PRIMER_LEFT_1=1444,23
PRIMER_RIGHT_1=2003,23
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=560
PRIMER_PAIR_PENALTY_2=0.1253
PRIMER_LEFT_2_PENALTY=0.085102
PRIMER_RIGHT_2_PENALTY=0.040223
PRIMER_LEFT_2_SEQUENCE=ATCCTGATGACAGTTTCCAGTGT
PRIMER_RIGHT_2_SEQUENCE=CTCCTTGATAGAACCCTGTTGTG
PRIMER_LEFT_2=54,23
PRIMER_RIGHT_2=546,23
PRIMER_LEFT_2_TM=59.915
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=493
PRIMER_PAIR_PENALTY_3=0.1257
PRIMER_LEFT_3_PENALTY=0.040223
PRIMER_RIGHT_3_PENALTY=0.085505
PRIMER_LEFT_3_SEQUENCE=CACAACAGGGTTCTATCAAGGAG
PRIMER_RIGHT_3_SEQUENCE=AGGAATTAGTTGAGTGGTGGTGA
PRIMER_LEFT_3=524,23
PRIMER_RIGHT_3=1466,23
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=943
PRIMER_PAIR_PENALTY_4=0.1554
PRIMER_LEFT_4_PENALTY=0.115144
PRIMER_RIGHT_4_PENALTY=0.040223
PRIMER_LEFT_4_SEQUENCE=GTCTACCTGGAATGAAACGAATG
PRIMER_RIGHT_4_SEQUENCE=CTCCTTGATAGAACCCTGTTGTG
PRIMER_LEFT_4=75,23
PRIMER_RIGHT_4=546,23
PRIMER_LEFT_4_TM=59.885
PRIMER_RIGHT_4_TM=60.040
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=472
PRIMER_PAIR_PENALTY_5=0.1554
PRIMER_LEFT_5_PENALTY=0.115144
PRIMER_RIGHT_5_PENALTY=0.040223
PRIMER_LEFT_5_SEQUENCE=TGTCTACCTGGAATGAAACGAAT
PRIMER_RIGHT_5_SEQUENCE=CTCCTTGATAGAACCCTGTTGTG
PRIMER_LEFT_5=74,23
PRIMER_RIGHT_5=546,23
PRIMER_LEFT_5_TM=59.885
PRIMER_RIGHT_5_TM=60.040
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.6000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=473
PRIMER_PAIR_PENALTY_6=0.1788
PRIMER_LEFT_6_PENALTY=0.150297
PRIMER_RIGHT_6_PENALTY=0.028502
PRIMER_LEFT_6_SEQUENCE=CATCTACAGTGCAACCAAGACAG
PRIMER_RIGHT_6_SEQUENCE=CCTTGCTTTTGTTCTGTGAGTCT
PRIMER_LEFT_6=1520,23
PRIMER_RIGHT_6=2003,23
PRIMER_LEFT_6_TM=59.850
PRIMER_RIGHT_6_TM=59.971
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.1000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=484
PRIMER_PAIR_PENALTY_7=0.1792
PRIMER_LEFT_7_PENALTY=0.150712
PRIMER_RIGHT_7_PENALTY=0.028502
PRIMER_LEFT_7_SEQUENCE=TCTGAGAAGGAAAGTACCCAACA
PRIMER_RIGHT_7_SEQUENCE=CCTTGCTTTTGTTCTGTGAGTCT
PRIMER_LEFT_7=1897,23
PRIMER_RIGHT_7=2003,23
PRIMER_LEFT_7_TM=60.151
PRIMER_RIGHT_7_TM=59.971
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.1000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=107
PRIMER_PAIR_PENALTY_8=0.1828
PRIMER_LEFT_8_PENALTY=0.142611
PRIMER_RIGHT_8_PENALTY=0.040223
PRIMER_LEFT_8_SEQUENCE=AGCGATTGTCTGGATAAACTGAA
PRIMER_RIGHT_8_SEQUENCE=CTCCTTGATAGAACCCTGTTGTG
PRIMER_LEFT_8=147,23
PRIMER_RIGHT_8=546,23
PRIMER_LEFT_8_TM=60.143
PRIMER_RIGHT_8_TM=60.040
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=400
PRIMER_PAIR_PENALTY_9=0.1828
PRIMER_LEFT_9_PENALTY=0.142611
PRIMER_RIGHT_9_PENALTY=0.040223
PRIMER_LEFT_9_SEQUENCE=GCGATTGTCTGGATAAACTGAAG
PRIMER_RIGHT_9_SEQUENCE=CTCCTTGATAGAACCCTGTTGTG
PRIMER_LEFT_9=148,23
PRIMER_RIGHT_9=546,23
PRIMER_LEFT_9_TM=60.143
PRIMER_RIGHT_9_TM=60.040
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=399
PRIMER_PAIR_PENALTY_10=0.2019
PRIMER_LEFT_10_PENALTY=0.116379
PRIMER_RIGHT_10_PENALTY=0.085505
PRIMER_LEFT_10_SEQUENCE=AATGGCCAAGATTTAAGCACATA
PRIMER_RIGHT_10_SEQUENCE=AGGAATTAGTTGAGTGGTGGTGA
PRIMER_LEFT_10=1128,23
PRIMER_RIGHT_10=1466,23
PRIMER_LEFT_10_TM=59.884
PRIMER_RIGHT_10_TM=59.914
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=5.6000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=339
PRIMER_PAIR_PENALTY_11=0.2229
PRIMER_LEFT_11_PENALTY=0.040223
PRIMER_RIGHT_11_PENALTY=0.182658
PRIMER_LEFT_11_SEQUENCE=CACAACAGGGTTCTATCAAGGAG
PRIMER_RIGHT_11_SEQUENCE=CAGATGTGTATCTGCAAGGTTGA
PRIMER_LEFT_11=524,23
PRIMER_RIGHT_11=1067,23
PRIMER_LEFT_11_TM=60.040
PRIMER_RIGHT_11_TM=60.183
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=544
PRIMER_PAIR_PENALTY_12=0.2277
PRIMER_LEFT_12_PENALTY=0.085102
PRIMER_RIGHT_12_PENALTY=0.142611
PRIMER_LEFT_12_SEQUENCE=ATCCTGATGACAGTTTCCAGTGT
PRIMER_RIGHT_12_SEQUENCE=TTCAGTTTATCCAGACAATCGCT
PRIMER_LEFT_12=54,23
PRIMER_RIGHT_12=169,23
PRIMER_LEFT_12_TM=59.915
PRIMER_RIGHT_12_TM=60.143
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.1000
PRIMER_RIGHT_12_END_STABILITY=9.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=116
PRIMER_PAIR_PENALTY_13=0.2277
PRIMER_LEFT_13_PENALTY=0.085102
PRIMER_RIGHT_13_PENALTY=0.142611
PRIMER_LEFT_13_SEQUENCE=ATCCTGATGACAGTTTCCAGTGT
PRIMER_RIGHT_13_SEQUENCE=CTTCAGTTTATCCAGACAATCGC
PRIMER_LEFT_13=54,23
PRIMER_RIGHT_13=170,23
PRIMER_LEFT_13_TM=59.915
PRIMER_RIGHT_13_TM=60.143
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=9.8000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=117
PRIMER_PAIR_PENALTY_14=0.2291
PRIMER_LEFT_14_PENALTY=0.040223
PRIMER_RIGHT_14_PENALTY=0.188926
PRIMER_LEFT_14_SEQUENCE=CACAACAGGGTTCTATCAAGGAG
PRIMER_RIGHT_14_SEQUENCE=GAAGGTCCATTGTGTGTGTTGTA
PRIMER_LEFT_14=524,23
PRIMER_RIGHT_14=796,23
PRIMER_LEFT_14_TM=60.040
PRIMER_RIGHT_14_TM=59.811
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.0000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=273
PRIMER_PAIR_PENALTY_15=0.2306
PRIMER_LEFT_15_PENALTY=0.040223
PRIMER_RIGHT_15_PENALTY=0.190334
PRIMER_LEFT_15_SEQUENCE=CACAACAGGGTTCTATCAAGGAG
PRIMER_RIGHT_15_SEQUENCE=GTAATACTGGGGGTACTGGCTCT
PRIMER_LEFT_15=524,23
PRIMER_RIGHT_15=641,23
PRIMER_LEFT_15_TM=60.040
PRIMER_RIGHT_15_TM=59.810
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, PAB Human pseudoautosomal boundary-like sequence.
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=118
PRIMER_PAIR_PENALTY_16=0.2312
PRIMER_LEFT_16_PENALTY=0.085102
PRIMER_RIGHT_16_PENALTY=0.146140
PRIMER_LEFT_16_SEQUENCE=ATCCTGATGACAGTTTCCAGTGT
PRIMER_RIGHT_16_SEQUENCE=CATTTCCTCCTTGATAGAACCCT
PRIMER_LEFT_16=54,23
PRIMER_RIGHT_16=552,23
PRIMER_LEFT_16_TM=59.915
PRIMER_RIGHT_16_TM=59.854
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.1000
PRIMER_RIGHT_16_END_STABILITY=9.1000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=499
PRIMER_PAIR_PENALTY_17=0.2316
PRIMER_LEFT_17_PENALTY=0.191420
PRIMER_RIGHT_17_PENALTY=0.040223
PRIMER_LEFT_17_SEQUENCE=GACAGAAGACAGCTTGAACCATT
PRIMER_RIGHT_17_SEQUENCE=CTCCTTGATAGAACCCTGTTGTG
PRIMER_LEFT_17=413,23
PRIMER_RIGHT_17=546,23
PRIMER_LEFT_17_TM=59.809
PRIMER_RIGHT_17_TM=60.040
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=134
PRIMER_PAIR_PENALTY_18=0.2316
PRIMER_LEFT_18_PENALTY=0.191420
PRIMER_RIGHT_18_PENALTY=0.040223
PRIMER_LEFT_18_SEQUENCE=ACAGAAGACAGCTTGAACCATTC
PRIMER_RIGHT_18_SEQUENCE=CTCCTTGATAGAACCCTGTTGTG
PRIMER_LEFT_18=414,23
PRIMER_RIGHT_18=546,23
PRIMER_LEFT_18_TM=59.809
PRIMER_RIGHT_18_TM=60.040
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=133
PRIMER_PAIR_PENALTY_19=0.2316
PRIMER_LEFT_19_PENALTY=0.146140
PRIMER_RIGHT_19_PENALTY=0.085505
PRIMER_LEFT_19_SEQUENCE=AGGGTTCTATCAAGGAGGAAATG
PRIMER_RIGHT_19_SEQUENCE=AGGAATTAGTTGAGTGGTGGTGA
PRIMER_LEFT_19=530,23
PRIMER_RIGHT_19=1466,23
PRIMER_LEFT_19_TM=59.854
PRIMER_RIGHT_19_TM=59.914
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=937
=