PRIMER_SEQUENCE_ID=NM_198316
SEQUENCE=GCTGGCCGTCTGCGCACCCTCTCTCCCCTCGGCTCTTTCCTAGGAAAGCTGAGCCTCATAGCTTCCGGGAGAAGGTTTTCCGGAAGAAACCTCCAGTCTGTGCAGTATGTAAGGTGACCATCGATGGGACAGGCGTTTCGTGCAGAGTCTGCAAGGTGGCGACGCACAGAAAATGTGAAGCAAAGGTGACTTCAGCCTGTCAGGCCTTGCCTCCCGTGGAGTTGCGGCGAAACACGGCCCCAGTCAGGCGCATAGAGCACCTGGGATCCACCAAATCTCTGAACCACTCAAAGCAGCGCAGCACTCTGCCCAGGAGCTTCAGCCTGGACCCGCTCATGGAGCGGCGCTGGGACTTAGACCTCACCTACGTGACGGAGCGCATCTTGGCCGCCGCCTTCCCCGCGCGGCCCGATGAACAGCGGCACCGGGGCCACCTGCGCGAGCTGGCCCATGTGCTGCAATCCAAGCACCGGGACAAGTACCTGCTCTTCAACCTTTCAGAGAAAAGGCATGACCTGACCCGCTTAAACCCCAAGGTTCAAGACTTCGGCTGGCCTGAGCTGCATGCTCCACCCCTGGACAAGCTGTGCTCCATCTGCAAAGCCATGGAGACATGGCTCAGTGCTGACCCACAGCACGTGGTCGTACTATACTGCAAGGGAAACAAGGGCAAGCTTGGGGTCATCGTTTCTGCCTACATGCACTACAGCAAGATCTCTGCAGGGGCGGACCAGGCACTGGCCACTCTTACCATGCGGAAATTCTGCGAGGACAAGGTGGCCACAGAACTGCAGCCCTCCCAGCGTCGATATATCAGCTACTTCAGTGGGCTGCTATCTGGCTCCATCAGAATGAACAGCAGCCCTCTCTTCCTGCACTATGTGCTCATCCCCATGCTGCCAGCCTTTGAACCTGGCACAGGCTTCCAGCCCTTCCTTAAAATCTACCAGTCCATGCAGCTTGTCTACACATCTGGAGTCTATCACATTGCAGGCCCTGGTCCCCAGCAGCTTTGCATCAGCCTGGAGCCAGCCCTCCTCCTCAAAGGCGATGTCATGGTAACATGTTATCACAAGGGTGGCCGGGGCACAGACCGGACCCTCGTGTTCCGAGTCCAGTTCCACACCTGCACCATCCACGGACCACAGCTCACTTTCCCCAAGGACCAGCTTGACGAGGCCTGGACTGATGAGAGGTTCCCCTTCCAAGCCTCCGTGGAGTTTGTCTTCTCCTCCAGCCCCGAGAAGATCAAAGGCAGCACTCCACGGAACGACCCCTCGGTCTCTGTCGACTACAACACCACTGAGCCAGCCGTGCGCTGGGACTCCTATGAGAACTTCAACCAGCACCACGAGGACAGTGTGGATGGCTCCTTGACCCACACCCGGGGTCCCCTGGATGGCAGTCCTTATGCCCAGGTGCAGCGGCCTCCCCGGCAGACCCCCCCGGCACCCTCTCCAGAGCCTCCACCACCCCCCATGCTCTCTGTCAGCAGCGACTCAGGCCATTCCTCCACGCTGACCACAGAGCCGGCTGCTGAGTCCCCTGGCCGGCCGCCCCCTACAGCTGCTGAACGGCAGGAGCTGGATCGCCTCCTAGGAGGCTGCGGAGTGGCCAGTGGGGGCCGGGGAGCTGGGCGCGAGACGGCCATCCTAGATGACGAAGAGCAGCCCACTGTGGGCGGAGGCCCCCACCTCGGAGTGTATCCAGGCCATAGGCCTGGCCTCAGCCGCCACTGCTCCTGCCGCCAGGGCTACCGGGAGCCCTGCGGGGTTCCCAATGGGGGCTACTACCGGCCAGAGGGAACCCTGGAGAGGAGGCGACTGGCCTACGGGGGCTATGAGGGATCCCCCCAGGGCTACGCCGAGGCCTCGATGGAGAAGAGGCGCCTCTGCCGATCGCTGTCAGAGGGGCTATACCCCTACCCACCTGAGATGGGGAAACCAGCCACTGGGGACTTTGGCTACCGCGCCCCAGGCTACCGGGAGGTGGTCATCCTGGAGGACCCTGGGCTGCCTGCCCTATACCCATGCCCAGCCTGCGAGGAGAAGCTGGCGCTGCCTACAGCAGCCTTGTATGGACTGCGGCTGGAGAGGGAGGCTGGAGAAGGGTGGGCAAGTGAGGCTGGCAAGCCTCTCCTGCACCCAGTGCGGCCTGGGCACCCGCTGCCTCTGCTCTTGCCTGCCTGTGGGCATCACCATGCCCCGATGCCTGACTACAGCTGCCTGAAGCCACCCAAGGCAGGCGAGGAAGGGCACGAGGGCTGCTCCTACACCATGTGCCCCGAAGGCAGGTATGGGCATCCAGGGTACCCTGCCCTGGTGACATACAGCTATGGAGGAGCAGTTCCCAGTTACTGCCCAGCATATGGCCGTGTGCCTCATAGCTGTGGCTCTCCAGGAGAGGGCAGAGGGTATCCCAGCCCTGGTGCCCACTCCCCACGGGCTGGCTCCATTTCCCCGGGCAGCCCGCCCTATCCACAATCTAGGAAGCTGAGCTACGAGATCCCTACGGAGGAGGGAGGGGACAGGTACCCATTGCCTGGGCACCTGGCCTCAGCAGGACCTTTGGCATCTGCAGAGTCGCTGGAGCCGGTGTCCTGGAGGGAGGGCCCCAGTGGGCACAGCACACTGCCTCGGTCTCCCCGAGATGCCCCATGCAGTGCTTCGTCAGAGTTGTCTGGTCCCTCCACGCCCCTGCACACCAGCAGTCCAGTCCAGGGCAAGGAAAGCACCCGGCGACAGGACACCAGGTCCCCCACCTCAGCGCCCACTCAGAGACTGAGTCCTGGCGAGGCCTTGCCCCCTGTTTCCCAGGCAGGCACCGGAAAGGCCCCTGAGCTGCCGTCGGGAAGTGGGCCTGAGCCTCTGGCCCCTAGCCCAGTCTCTCCGACCTTCCCTCCCAGCTCGCCCAGTGACTGGCCTCAGGAAAGGAGTCCAGGGGGCCACTCAGATGGCGCCAGTCCTCGGAGCCCTGTGCCCACCACACTTCCTGGCCTCCGCCACGCCCCCTGGCAAGGCCCTCGAGGCCCCCCCGACAGCCCAGATGGGTCTCCCCTCACTCCTGTGCCTTCCCAGATGCCCTGGCTTGTGGCCAGCCCAGAGCCGCCTCAGAGCTCACCTACACCTGCTTTCCCCCTGGCTGCCTCCTATGACACCAATGGCCTTAGCCAGCCCCCACTTCCTGAGAAACGCCACCTGCCCGGGCCGGGGCAACAGCCAGGACCCTGGGGCCCAGAGCAGGCATCATCGCCAGCCAGAGGCATCAGTCACCATGTCACCTTCGCACCTCTGCTCTCAGATAATGTCCCCCAAACCCCAGAGCCTCCTACACAAGAGAGCCAAAGCAATGTCAAGTTTGTCCAGGATACATCCAAGTTCTGGTACAAGCCACACCTGTCCCGTGACCAAGCCATTGCCCTGCTGAAGGACAAGGACCCTGGGGCCTTCCTGATCAGGGACAGTCATTCATTCCAAGGAGCTTATGGGCTGGCCCTCAAGGTGGCCACACCGCCACCCAGTGCCCAGCCCTGGAAAGGGGACCCCGTGGAACAGCTGGTCCGCCATTTCCTCATCGAGACTGGGCCCAAAGGGGTGAAGATCAAGGGCTGCCCCAGTGAGCCCTACTTTGGCAGCCTGTCCGCCTTGGTCTCCCAGCACTCCATCTCCCCCATCTCCCTGCCCTGCTGCCTGCGCATTCCCAGCAAAGATCCTCTGGAAGAGACCCCAGAGGCTCCAGTGCCCACCAACATGAGCACAGCGGCAGACCTCCTGCGTCAGGGTGCTGCCTGCAGCGTGCTCTACTTGACCTCAGTGGAGACAGAGTCACTGACGGGCCCCCAAGCTGTGGCCCGGGCCAGCTCTGCAGCTCTGAGCTGTAGCCCCCGCCCGACACCAGCTGTTGTCCACTTCAAGGTGTCAGCCCAGGGCATTACACTGACGGACAACCAAAGGAAGCTCTTCTTTCGCCGCCATTATCCAGTGAACAGCATCACCTTCTCCAGCACTGACCCTCAAGACCGGAGATGGACCAACCCAGACGGGACCACCTCCAAGATCTTTGGTTTCGTGGCCAAGAAGCCGGGAAGCCCCTGGGAGAATGTGTGTCACCTCTTTGCAGAGCTTGACCCAGATCAGCCTGCTGGCGCCATTGTCACCTTCATCACCAAAGTTCTACTGGGCCAGAGAAAATGAAGGAAGGCCACAAGCTCAGAGCCCACATCAACACTGCCCCCCTCCCAGCACCCCACAGCCCTCACATCCCCTGGCCTGGACCCAGGAGACCCAGGAGAAAGCACCCTCCCTTAGGAATGAGGAGTGGGCATCAGGCCTGGGACACTGCTCTCCTTCCCCGCCCCCAGCCTGCTAAGTTAAGTGGACAGGCCCACAAGATGACCTTGCATGTGAGCAGATGGCAGAGATGGGTGTGTGAGGGGTGAGGAGGCATCAGCAGTTGAGCCCCGAAGGAGATCAGGCAGCCCCACCTGCAGGAGAACGTCAGCCCTCCAGGGGATCAGCCCCTGCCAGTTCCACCCAGCTGCAGGTGCCAGCACGGCAGGGATGGGAGAGGGGTGGGGAGCGAGTCACTGCCTCCTCTGAGCAGAGATTCAGAGTAGGATCACATGAATAGGGGAAAAAAGAGAGTCTATTTTTGTCTAATAATAAAGAATTTCTATAAACTTTAGCCGAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 54398, GC content failed 1515, low tm 10589, high tm 32174, high any compl 2, high end compl 39, high repeat similarity 29, long poly-x seq 98, ok 9952
PRIMER_RIGHT_EXPLAIN=considered 54257, GC content failed 1803, low tm 10744, high tm 31717, high any compl 1, high end compl 16, high repeat similarity 21, long poly-x seq 113, ok 9842
PRIMER_PAIR_EXPLAIN=considered 1031, unacceptable product size 978, high end compl 4, ok 49
PRIMER_PAIR_PENALTY=0.0571
PRIMER_LEFT_PENALTY=0.038744
PRIMER_RIGHT_PENALTY=0.018314
PRIMER_LEFT_SEQUENCE=GTGGTCGTACTATACTGCAAGGG
PRIMER_RIGHT_SEQUENCE=GTAGATTTTAAGGAAGGGCTGGA
PRIMER_LEFT=638,23
PRIMER_RIGHT=946,23
PRIMER_LEFT_TM=59.961
PRIMER_RIGHT_TM=59.982
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=309
PRIMER_PAIR_PENALTY_1=0.0965
PRIMER_LEFT_1_PENALTY=0.078208
PRIMER_RIGHT_1_PENALTY=0.018314
PRIMER_LEFT_1_SEQUENCE=CTTAAACCCCAAGGTTCAAGACT
PRIMER_RIGHT_1_SEQUENCE=GTAGATTTTAAGGAAGGGCTGGA
PRIMER_LEFT_1=523,23
PRIMER_RIGHT_1=946,23
PRIMER_LEFT_1_TM=59.922
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=424
PRIMER_PAIR_PENALTY_2=0.1212
PRIMER_LEFT_2_PENALTY=0.102866
PRIMER_RIGHT_2_PENALTY=0.018314
PRIMER_LEFT_2_SEQUENCE=CTGTGCAGTATGTAAGGTGACCA
PRIMER_RIGHT_2_SEQUENCE=GTAGATTTTAAGGAAGGGCTGGA
PRIMER_LEFT_2=97,23
PRIMER_RIGHT_2=946,23
PRIMER_LEFT_2_TM=60.103
PRIMER_RIGHT_2_TM=59.982
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=850
PRIMER_PAIR_PENALTY_3=0.1379
PRIMER_LEFT_3_PENALTY=0.018314
PRIMER_RIGHT_3_PENALTY=0.119617
PRIMER_LEFT_3_SEQUENCE=TCCAGCCCTTCCTTAAAATCTAC
PRIMER_RIGHT_3_SEQUENCE=CAGTGGTGTTGTAGTCGACAGAG
PRIMER_LEFT_3=924,23
PRIMER_RIGHT_3=1304,23
PRIMER_LEFT_3_TM=59.982
PRIMER_RIGHT_3_TM=59.880
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=381
PRIMER_PAIR_PENALTY_4=0.1379
PRIMER_LEFT_4_PENALTY=0.018314
PRIMER_RIGHT_4_PENALTY=0.119617
PRIMER_LEFT_4_SEQUENCE=TCCAGCCCTTCCTTAAAATCTAC
PRIMER_RIGHT_4_SEQUENCE=CTCAGTGGTGTTGTAGTCGACAG
PRIMER_LEFT_4=924,23
PRIMER_RIGHT_4=1306,23
PRIMER_LEFT_4_TM=59.982
PRIMER_RIGHT_4_TM=59.880
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=383
PRIMER_PAIR_PENALTY_5=0.1584
PRIMER_LEFT_5_PENALTY=0.038744
PRIMER_RIGHT_5_PENALTY=0.119617
PRIMER_LEFT_5_SEQUENCE=GTGGTCGTACTATACTGCAAGGG
PRIMER_RIGHT_5_SEQUENCE=CAGTGGTGTTGTAGTCGACAGAG
PRIMER_LEFT_5=638,23
PRIMER_RIGHT_5=1304,23
PRIMER_LEFT_5_TM=59.961
PRIMER_RIGHT_5_TM=59.880
PRIMER_LEFT_5_GC_PERCENT=52.174
PRIMER_RIGHT_5_GC_PERCENT=52.174
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=667
PRIMER_PAIR_PENALTY_6=0.1584
PRIMER_LEFT_6_PENALTY=0.038744
PRIMER_RIGHT_6_PENALTY=0.119617
PRIMER_LEFT_6_SEQUENCE=GTGGTCGTACTATACTGCAAGGG
PRIMER_RIGHT_6_SEQUENCE=CTCAGTGGTGTTGTAGTCGACAG
PRIMER_LEFT_6=638,23
PRIMER_RIGHT_6=1306,23
PRIMER_LEFT_6_TM=59.961
PRIMER_RIGHT_6_TM=59.880
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=52.174
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=669
PRIMER_PAIR_PENALTY_7=0.1978
PRIMER_LEFT_7_PENALTY=0.078208
PRIMER_RIGHT_7_PENALTY=0.119617
PRIMER_LEFT_7_SEQUENCE=CTTAAACCCCAAGGTTCAAGACT
PRIMER_RIGHT_7_SEQUENCE=CAGTGGTGTTGTAGTCGACAGAG
PRIMER_LEFT_7=523,23
PRIMER_RIGHT_7=1304,23
PRIMER_LEFT_7_TM=59.922
PRIMER_RIGHT_7_TM=59.880
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=52.174
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.1000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=782
PRIMER_PAIR_PENALTY_8=0.1978
PRIMER_LEFT_8_PENALTY=0.078208
PRIMER_RIGHT_8_PENALTY=0.119617
PRIMER_LEFT_8_SEQUENCE=CTTAAACCCCAAGGTTCAAGACT
PRIMER_RIGHT_8_SEQUENCE=CTCAGTGGTGTTGTAGTCGACAG
PRIMER_LEFT_8=523,23
PRIMER_RIGHT_8=1306,23
PRIMER_LEFT_8_TM=59.922
PRIMER_RIGHT_8_TM=59.880
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=52.174
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.1000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=784
PRIMER_PAIR_PENALTY_9=0.2084
PRIMER_LEFT_9_PENALTY=0.032744
PRIMER_RIGHT_9_PENALTY=0.175636
PRIMER_LEFT_9_SEQUENCE=GGTGACATACAGCTATGGAGGAG
PRIMER_RIGHT_9_SEQUENCE=TCGTAGCTCAGCTTCCTAGATTG
PRIMER_LEFT_9=2320,23
PRIMER_RIGHT_9=2502,23
PRIMER_LEFT_9_TM=60.033
PRIMER_RIGHT_9_TM=60.176
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MIR2 Repetitive element - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=183
PRIMER_PAIR_PENALTY_10=0.2486
PRIMER_LEFT_10_PENALTY=0.072785
PRIMER_RIGHT_10_PENALTY=0.175845
PRIMER_LEFT_10_SEQUENCE=GCACCTCTGCTCTCAGATAATGT
PRIMER_RIGHT_10_SEQUENCE=AAAGAAGAGCTTCCTTTGGTTGT
PRIMER_LEFT_10=3284,23
PRIMER_RIGHT_10=3964,23
PRIMER_LEFT_10_TM=59.927
PRIMER_RIGHT_10_TM=59.824
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=8.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=681
PRIMER_PAIR_PENALTY_11=0.2978
PRIMER_LEFT_11_PENALTY=0.175636
PRIMER_RIGHT_11_PENALTY=0.122120
PRIMER_LEFT_11_SEQUENCE=CAATCTAGGAAGCTGAGCTACGA
PRIMER_RIGHT_11_SEQUENCE=CAGAACTTGGATGTATCCTGGAC
PRIMER_LEFT_11=2480,23
PRIMER_RIGHT_11=3381,23
PRIMER_LEFT_11_TM=60.176
PRIMER_RIGHT_11_TM=59.878
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.4000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=902
PRIMER_PAIR_PENALTY_12=0.2980
PRIMER_LEFT_12_PENALTY=0.122120
PRIMER_RIGHT_12_PENALTY=0.175845
PRIMER_LEFT_12_SEQUENCE=TGTCCAGGATACATCCAAGTTCT
PRIMER_RIGHT_12_SEQUENCE=AAAGAAGAGCTTCCTTTGGTTGT
PRIMER_LEFT_12=3358,23
PRIMER_RIGHT_12=3964,23
PRIMER_LEFT_12_TM=59.878
PRIMER_RIGHT_12_TM=59.824
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=8.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=607
PRIMER_PAIR_PENALTY_13=0.3223
PRIMER_LEFT_13_PENALTY=0.304014
PRIMER_RIGHT_13_PENALTY=0.018314
PRIMER_LEFT_13_SEQUENCE=GAAACCTCCAGTCTGTGCAGTAT
PRIMER_RIGHT_13_SEQUENCE=GTAGATTTTAAGGAAGGGCTGGA
PRIMER_LEFT_13=85,23
PRIMER_RIGHT_13=946,23
PRIMER_LEFT_13_TM=59.696
PRIMER_RIGHT_13_TM=59.982
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.3000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=862
PRIMER_PAIR_PENALTY_14=0.3527
PRIMER_LEFT_14_PENALTY=0.072785
PRIMER_RIGHT_14_PENALTY=0.279943
PRIMER_LEFT_14_SEQUENCE=GCACCTCTGCTCTCAGATAATGT
PRIMER_RIGHT_14_SEQUENCE=AAGAAGAGCTTCCTTTGGTTGTC
PRIMER_LEFT_14=3284,23
PRIMER_RIGHT_14=3963,23
PRIMER_LEFT_14_TM=59.927
PRIMER_RIGHT_14_TM=60.280
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=680
PRIMER_PAIR_PENALTY_15=0.3578
PRIMER_LEFT_15_PENALTY=0.182211
PRIMER_RIGHT_15_PENALTY=0.175636
PRIMER_LEFT_15_SEQUENCE=CATACAGCTATGGAGGAGCAGTT
PRIMER_RIGHT_15_SEQUENCE=TCGTAGCTCAGCTTCCTAGATTG
PRIMER_LEFT_15=2325,23
PRIMER_RIGHT_15=2502,23
PRIMER_LEFT_15_TM=59.818
PRIMER_RIGHT_15_TM=60.176
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=178
PRIMER_PAIR_PENALTY_16=0.3890
PRIMER_LEFT_16_PENALTY=0.356216
PRIMER_RIGHT_16_PENALTY=0.032744
PRIMER_LEFT_16_SEQUENCE=CTATACCCCTACCCACCTGAGAT
PRIMER_RIGHT_16_SEQUENCE=CTCCTCCATAGCTGTATGTCACC
PRIMER_LEFT_16=1913,23
PRIMER_RIGHT_16=2342,23
PRIMER_LEFT_16_TM=59.644
PRIMER_RIGHT_16_TM=60.033
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MIR2 Repetitive element - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.3000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=430
PRIMER_PAIR_PENALTY_17=0.3940
PRIMER_LEFT_17_PENALTY=0.072785
PRIMER_RIGHT_17_PENALTY=0.321265
PRIMER_LEFT_17_SEQUENCE=GCACCTCTGCTCTCAGATAATGT
PRIMER_RIGHT_17_SEQUENCE=CTTTGGTTGTCCGTCAGTGTAAT
PRIMER_LEFT_17=3284,23
PRIMER_RIGHT_17=3951,23
PRIMER_LEFT_17_TM=59.927
PRIMER_RIGHT_17_TM=60.321
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=5.6000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=668
PRIMER_PAIR_PENALTY_18=0.4021
PRIMER_LEFT_18_PENALTY=0.122120
PRIMER_RIGHT_18_PENALTY=0.279943
PRIMER_LEFT_18_SEQUENCE=TGTCCAGGATACATCCAAGTTCT
PRIMER_RIGHT_18_SEQUENCE=AAGAAGAGCTTCCTTTGGTTGTC
PRIMER_LEFT_18=3358,23
PRIMER_RIGHT_18=3963,23
PRIMER_LEFT_18_TM=59.878
PRIMER_RIGHT_18_TM=60.280
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=606
PRIMER_PAIR_PENALTY_19=0.4109
PRIMER_LEFT_19_PENALTY=0.235024
PRIMER_RIGHT_19_PENALTY=0.175845
PRIMER_LEFT_19_SEQUENCE=TTGTCCAGGATACATCCAAGTTC
PRIMER_RIGHT_19_SEQUENCE=AAAGAAGAGCTTCCTTTGGTTGT
PRIMER_LEFT_19=3357,23
PRIMER_RIGHT_19=3964,23
PRIMER_LEFT_19_TM=60.235
PRIMER_RIGHT_19_TM=59.824
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=608
=