PRIMER PICKING RESULTS FOR NM_000061,Homo sapiens Bruton agammaglobulinemia tyrosine kinase (BTK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1667   20   59.99   50.00  4.00  2.00 11.00 TGCAAGGATGTCTGTGAAGC
RIGHT PRIMER      2078   20   59.99   45.00  4.00  0.00 12.00 CTGCTTTCTCATGCCAACAA
SEQUENCE SIZE: 2591
INCLUDED REGION SIZE: 2591

PRODUCT SIZE: 412, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       1443   20   60.01   40.00  6.00  2.00 11.00 TGGCCATCAAGATGATCAAA
   RIGHT PRIMER      2078   20   59.99   45.00  4.00  0.00 12.00 CTGCTTTCTCATGCCAACAA
   PRODUCT SIZE: 636, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1706   20   59.99   50.00  4.00  3.00 11.00 AAGCAGTTCCTTCACCGAGA
   RIGHT PRIMER      2078   20   59.99   45.00  4.00  0.00 12.00 CTGCTTTCTCATGCCAACAA
   PRODUCT SIZE: 373, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1602   20   60.01   60.00  3.00  1.00 11.00 GCTGCCTCCTGAACTACCTG
   RIGHT PRIMER      2078   20   59.99   45.00  4.00  0.00 12.00 CTGCTTTCTCATGCCAACAA
   PRODUCT SIZE: 477, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       1667   20   59.99   50.00  4.00  2.00 11.00 TGCAAGGATGTCTGTGAAGC
   RIGHT PRIMER      2029   20   59.97   55.00  6.00  2.00 10.00 AGCCAGATGAGGCCTGTAGA
   PRODUCT SIZE: 363, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00