PRIMER PICKING RESULTS FOR NM_000141,Homo sapiens fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss syndrome) (FGFR2), transcript variant 1, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2260   20   60.03   55.00  4.00  3.00 10.00 GGATGGGCCTCTCTATGTCA
RIGHT PRIMER      2847   20   60.02   50.00  6.00  1.00 11.00 TGCTTGAACGTTGGTCTCTG
SEQUENCE SIZE: 4587
INCLUDED REGION SIZE: 4587

PRODUCT SIZE: 588, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       3636   20   60.05   40.00  5.00  0.00 11.00 TTTGCTTTCAGGGAAAATGG
   RIGHT PRIMER      3889   20   60.02   50.00  5.00  3.00 11.00 CAATCGTCTGACAGCAGCAT
   PRODUCT SIZE: 254, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER       3004   20   60.07   50.00  4.00  2.00 12.00 TTACGAACCATGCCTTCCTC
   RIGHT PRIMER      3889   20   60.02   50.00  5.00  3.00 11.00 CAATCGTCTGACAGCAGCAT
   PRODUCT SIZE: 886, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       1974   20   60.07   55.00  5.00  0.00 10.00 CAGGGGTCTCCGAGTATGAA
   RIGHT PRIMER      2847   20   60.02   50.00  6.00  1.00 11.00 TGCTTGAACGTTGGTCTCTG
   PRODUCT SIZE: 874, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       2828   20   60.02   50.00  6.00  0.00 12.00 CAGAGACCAACGTTCAAGCA
   RIGHT PRIMER      3023   20   60.07   50.00  4.00  2.00 11.00 GAGGAAGGCATGGTTCGTAA
   PRODUCT SIZE: 196, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00