PRIMER PICKING RESULTS FOR NM_000142,Homo sapiens fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) (FGFR3), transcript variant 1, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1567   20   59.98   50.00  3.00  1.00 10.00 CTGAAAGACGATGCCACTGA
RIGHT PRIMER      1825   20   60.00   55.00  7.00  3.00 11.00 ACACCAGGTCCTTGAAGGTG
SEQUENCE SIZE: 4093
INCLUDED REGION SIZE: 4093

PRODUCT SIZE: 259, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER        987   20   60.01   60.00  4.00  0.00 11.00 CACCGACAAGGAGCTAGAGG
   RIGHT PRIMER      1098   20   59.98   50.00  5.00  0.00 11.00 CCACGCAGAGTGATGAGAAA
   PRODUCT SIZE: 112, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER        987   20   60.01   60.00  4.00  0.00 11.00 CACCGACAAGGAGCTAGAGG
   RIGHT PRIMER      1586   20   59.98   50.00  3.00  1.00 10.00 TCAGTGGCATCGTCTTTCAG
   PRODUCT SIZE: 600, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        986   20   60.01   60.00  4.00  2.00 11.00 CCACCGACAAGGAGCTAGAG
   RIGHT PRIMER      1098   20   59.98   50.00  5.00  0.00 11.00 CCACGCAGAGTGATGAGAAA
   PRODUCT SIZE: 113, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER        986   20   60.01   60.00  4.00  2.00 11.00 CCACCGACAAGGAGCTAGAG
   RIGHT PRIMER      1586   20   59.98   50.00  3.00  1.00 10.00 TCAGTGGCATCGTCTTTCAG
   PRODUCT SIZE: 601, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00