PRIMER PICKING RESULTS FOR NM_000208,Homo sapiens insulin receptor (INSR), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        271   20   59.98   50.00  4.00  2.00 10.00 CATCGAAGGACACTTGCAGA
RIGHT PRIMER      1044   20   60.01   50.00  4.00  0.00 10.00 GGGATGCACTTGTTGTTGTG
SEQUENCE SIZE: 4691
INCLUDED REGION SIZE: 4691

PRODUCT SIZE: 774, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER        270   20   59.98   50.00  4.00  2.00 10.00 TCATCGAAGGACACTTGCAG
   RIGHT PRIMER      1044   20   60.01   50.00  4.00  0.00 10.00 GGGATGCACTTGTTGTTGTG
   PRODUCT SIZE: 775, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER       3073   20   60.02   45.00  3.00  3.00 10.00 ACCGCTTTACGCTTCTTCAA
   RIGHT PRIMER      3817   20   60.02   55.00  4.00  2.00 10.00 GTAAGGCTGTTCTGCCAAGC
   PRODUCT SIZE: 745, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       3109   20   59.97   45.00  3.00  2.00 12.00 TGCCAGTGATGTGTTTCCAT
   RIGHT PRIMER      3817   20   60.02   55.00  4.00  2.00 10.00 GTAAGGCTGTTCTGCCAAGC
   PRODUCT SIZE: 709, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       3111   20   59.97   50.00  4.00  2.00 12.00 CCAGTGATGTGTTTCCATGC
   RIGHT PRIMER      3817   20   60.02   55.00  4.00  2.00 10.00 GTAAGGCTGTTCTGCCAAGC
   PRODUCT SIZE: 707, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00