PRIMER PICKING RESULTS FOR NM_000215,Homo sapiens Janus kinase 3 (a protein tyrosine kinase, leukocyte) (JAK3), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2714   20   59.99   50.00  5.00  2.00 11.00 GATCCTCAAAGCACTGCACA
RIGHT PRIMER      3462   20   60.00   55.00  3.00  0.00 11.00 AGGACAGGGAGTGGTGTTTG
SEQUENCE SIZE: 4064
INCLUDED REGION SIZE: 4064

PRODUCT SIZE: 749, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       3443   20   60.00   55.00  3.00  0.00 12.00 CAAACACCACTCCCTGTCCT
   RIGHT PRIMER      3592   20   59.96   55.00  4.00  0.00 11.00 TGGGGGTGTTCCTGAAGTAG
   PRODUCT SIZE: 150, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER       1244   20   59.99   45.00  4.00  1.00 11.00 CTTTGCCATCAACAAGCTCA
   RIGHT PRIMER      1559   20   59.97   55.00  4.00  1.00 11.00 GACCACGATCAGGTTGGACT
   PRODUCT SIZE: 316, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       1246   20   59.99   45.00  4.00  2.00 12.00 TTGCCATCAACAAGCTCAAG
   RIGHT PRIMER      1559   20   59.97   55.00  4.00  1.00 11.00 GACCACGATCAGGTTGGACT
   PRODUCT SIZE: 314, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       1244   20   59.99   45.00  4.00  1.00 11.00 CTTTGCCATCAACAAGCTCA
   RIGHT PRIMER      1557   20   59.97   50.00  4.00  0.00 11.00 CCACGATCAGGTTGGACTTT
   PRODUCT SIZE: 314, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00