PRIMER PICKING RESULTS FOR NM_000222,Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       3948   20   59.97   50.00  3.00  1.00 12.00 GTGGGAAAACACTGCCATCT
RIGHT PRIMER      4633   20   59.98   55.00  3.00  0.00 11.00 GGAAAGGTGCGAGAGCATAG
SEQUENCE SIZE: 5084
INCLUDED REGION SIZE: 5084

PRODUCT SIZE: 686, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       3947   20   59.97   50.00  4.00  1.00 11.00 AGTGGGAAAACACTGCCATC
   RIGHT PRIMER      4633   20   59.98   55.00  3.00  0.00 11.00 GGAAAGGTGCGAGAGCATAG
   PRODUCT SIZE: 687, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER       1575   20   60.00   55.00  3.00  0.00 10.00 CCACACCCTGTTCACTCCTT
   RIGHT PRIMER      2443   20   60.05   45.00  5.00  3.00  9.00 TCTTTGTGATCCGACCATGA
   PRODUCT SIZE: 869, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1575   20   60.00   55.00  3.00  0.00 10.00 CCACACCCTGTTCACTCCTT
   RIGHT PRIMER      2115   20   59.95   45.00  5.00  0.00 11.00 TGCATGATCTTCCTGCTTTG
   PRODUCT SIZE: 541, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER         45   20   59.96   50.00  2.00  2.00 11.00 GGATTTTCTCTGCGTTCTGC
   RIGHT PRIMER       616   20   60.02   50.00  4.00  1.00 10.00 TTTCCGACAGCACTGACTTG
   PRODUCT SIZE: 572, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00