PRIMER PICKING RESULTS FOR NM_000245,Homo sapiens met proto-oncogene (hepatocyte growth factor receptor) (MET), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       3497   20   59.97   45.00  3.00  0.00 11.00 AAGAGGGCATTTTGGTTGTG
RIGHT PRIMER      3632   20   60.01   50.00  3.00  1.00 11.00 GATGATTCCCTCGGTCAGAA
SEQUENCE SIZE: 4620
INCLUDED REGION SIZE: 4620

PRODUCT SIZE: 136, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER        685   20   60.01   55.00  3.00  1.00 11.00 TAGAAGAGCCCAGCCAGTGT
   RIGHT PRIMER       827   20   60.03   45.00  5.00  1.00 10.00 CGAATGCAATGGATGATCTG
   PRODUCT SIZE: 143, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER       3613   20   60.01   50.00  3.00  1.00 10.00 TTCTGACCGAGGGAATCATC
   RIGHT PRIMER      4339   20   59.96   50.00  2.00  2.00 11.00 GGATACGGAGCGACACATTT
   PRODUCT SIZE: 727, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       3613   20   60.01   50.00  3.00  1.00 10.00 TTCTGACCGAGGGAATCATC
   RIGHT PRIMER      4387   20   59.96   55.00  4.00  1.00 10.00 CGTGTGTCCACCTCATCATC
   PRODUCT SIZE: 775, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       3613   20   60.01   50.00  3.00  1.00 10.00 TTCTGACCGAGGGAATCATC
   RIGHT PRIMER      4388   20   59.96   50.00  4.00  3.00 10.00 TCGTGTGTCCACCTCATCAT
   PRODUCT SIZE: 776, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00