PRIMER PICKING RESULTS FOR NM_000459,Homo sapiens TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal) (TEK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        675   20   60.01   55.00  4.00  3.00 10.00 TACACCTGCCTCATGCTCAG
RIGHT PRIMER      1518   20   60.00   50.00  4.00  2.00 10.00 CCAGTGTCAATCACGTTTGG
SEQUENCE SIZE: 4138
INCLUDED REGION SIZE: 4138

PRODUCT SIZE: 844, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       1499   20   60.00   50.00  4.00  1.00 10.00 CCAAACGTGATTGACACTGG
   RIGHT PRIMER      1759   20   60.03   55.00  8.00  1.00 12.00 TGTGAAGCGTCTCACAGGTC
   PRODUCT SIZE: 261, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER        675   20   60.01   55.00  4.00  3.00 10.00 TACACCTGCCTCATGCTCAG
   RIGHT PRIMER       916   20   60.02   50.00  5.00  2.00 12.00 TTCACAAGCCTTCTCACACG
   PRODUCT SIZE: 242, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER        834   20   59.97   45.00  4.00  1.00 11.00 ACAATGGTGTCTGCCATGAA
   RIGHT PRIMER      1518   20   60.00   50.00  4.00  2.00 10.00 CCAGTGTCAATCACGTTTGG
   PRODUCT SIZE: 685, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER        833   20   59.97   45.00  4.00  3.00 11.00 AACAATGGTGTCTGCCATGA
   RIGHT PRIMER      1518   20   60.00   50.00  4.00  2.00 10.00 CCAGTGTCAATCACGTTTGG
   PRODUCT SIZE: 686, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00