PRIMER PICKING RESULTS FOR NM_000875,Homo sapiens insulin-like growth factor 1 receptor (IGF1R), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2952   20   60.01   55.00  3.00  1.00 12.00 TGGAGTGCTGTATGCCTCTG
RIGHT PRIMER      3369   20   60.01   60.00  6.00  2.00 11.00 GCTTGGAGGTGCTAGGACTG
SEQUENCE SIZE: 4989
INCLUDED REGION SIZE: 4989

PRODUCT SIZE: 418, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       2153   20   59.99   50.00  2.00  0.00 11.00 CCAAAACTGAAGCCGAGAAG
   RIGHT PRIMER      2971   20   60.01   55.00  3.00  0.00 12.00 CAGAGGCATACAGCACTCCA
   PRODUCT SIZE: 819, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       2153   20   59.99   50.00  2.00  0.00 11.00 CCAAAACTGAAGCCGAGAAG
   RIGHT PRIMER      2675   20   59.98   50.00  5.00  3.00 11.00 CGCTGATCCTCAACTTGTGA
   PRODUCT SIZE: 523, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        462   20   60.01   45.00  4.00  0.00 12.00 GGCCATCAGGATTGAGAAAA
   RIGHT PRIMER      1331   20   60.02   50.00  4.00  2.00 11.00 TGCAAGTTCTGGTTGTCGAG
   PRODUCT SIZE: 870, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       4360   20   59.97   50.00  4.00  1.00 12.00 TGCCCAAACCCTTAACTGAC
   RIGHT PRIMER      4840   20   59.98   50.00  3.00  3.00 10.00 CAGCGTGTCTCTCAAATGGA
   PRODUCT SIZE: 481, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00