PRIMER PICKING RESULTS FOR NM_001715,Homo sapiens B lymphoid tyrosine kinase (BLK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        100   20   60.02   50.00  4.00  3.00 10.00 TTTGCTAACTCTGCCGGACT
RIGHT PRIMER       268   20   59.99   50.00  5.00  2.00 10.00 TCTGCTCCAGGAAAACTCGT
SEQUENCE SIZE: 2642
INCLUDED REGION SIZE: 2642

PRODUCT SIZE: 169, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       1414   20   59.98   55.00  7.00  0.00 10.00 CAGAAGCCTTTCTGGGTGAG
   RIGHT PRIMER      1852   20   60.01   55.00  5.00  0.00 10.00 AGGACTCCAAACGACCACAC
   PRODUCT SIZE: 439, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER        249   20   59.99   50.00  5.00  2.00 11.00 ACGAGTTTTCCTGGAGCAGA
   RIGHT PRIMER       497   20   59.97   50.00  3.00  0.00 12.00 GCAACTTCCAACACCATCCT
   PRODUCT SIZE: 249, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        249   20   59.99   50.00  5.00  2.00 11.00 ACGAGTTTTCCTGGAGCAGA
   RIGHT PRIMER       498   20   59.97   50.00  3.00  0.00 11.00 AGCAACTTCCAACACCATCC
   PRODUCT SIZE: 250, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER        100   20   60.02   50.00  4.00  3.00 10.00 TTTGCTAACTCTGCCGGACT
   RIGHT PRIMER       497   20   59.97   50.00  3.00  0.00 12.00 GCAACTTCCAACACCATCCT
   PRODUCT SIZE: 398, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00