PRIMER PICKING RESULTS FOR NM_001721,Homo sapiens BMX non-receptor tyrosine kinase (BMX), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1517   20   60.03   55.00  4.00  2.00 10.00 GGAGGCCCAGACTATGATGA
RIGHT PRIMER      1883   20   60.02   55.00  4.00  2.00 12.00 TGGAGCTGACCACTTGACTG
SEQUENCE SIZE: 2553
INCLUDED REGION SIZE: 2553

PRODUCT SIZE: 367, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       1990   20   60.00   55.00  5.00  2.00 12.00 ACAACTCCCAGGTGGTTCTG
   RIGHT PRIMER      2161   20   60.08   45.00  4.00  0.00 12.00 TTGTCTTTTTCCCGAAGTGG
   PRODUCT SIZE: 172, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1990   20   60.00   55.00  5.00  2.00 12.00 ACAACTCCCAGGTGGTTCTG
   RIGHT PRIMER      2157   20   60.08   45.00  4.00  1.00  9.00 CTTTTTCCCGAAGTGGTTCA
   PRODUCT SIZE: 168, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       1864   20   60.02   55.00  4.00  1.00 10.00 CAGTCAAGTGGTCAGCTCCA
   RIGHT PRIMER      2157   20   60.08   45.00  4.00  1.00  9.00 CTTTTTCCCGAAGTGGTTCA
   PRODUCT SIZE: 294, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       1864   20   60.02   55.00  4.00  1.00 10.00 CAGTCAAGTGGTCAGCTCCA
   RIGHT PRIMER      2161   20   60.08   45.00  4.00  0.00 12.00 TTGTCTTTTTCCCGAAGTGG
   PRODUCT SIZE: 298, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00