PRIMER PICKING RESULTS FOR NM_001982,Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) (ERBB3), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        428   20   59.98   50.00  4.00  0.00 10.00 TGCAGTGGATTCGAGAAGTG
RIGHT PRIMER      1371   20   59.99   55.00  7.00  2.00 10.00 CCGTACTGTCCGGAAGACAT
SEQUENCE SIZE: 4975
INCLUDED REGION SIZE: 4975

PRODUCT SIZE: 944, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       3839   20   59.98   55.00  4.00  2.00 11.00 CAAGTTCCCTTGAGGAGCTG
   RIGHT PRIMER      4369   20   60.01   45.00  5.00  3.00 10.00 GCCTCCAAAGATTGAATGGA
   PRODUCT SIZE: 531, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       2177   20   60.02   55.00  3.00  1.00 10.00 GCGGCACTTTTCTCTACTGG
   RIGHT PRIMER      2770   20   59.97   50.00  3.00  2.00 12.00 GGTCAGCCACACCAAAATCT
   PRODUCT SIZE: 594, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       2176   20   60.02   55.00  3.00  1.00 11.00 GGCGGCACTTTTCTCTACTG
   RIGHT PRIMER      2770   20   59.97   50.00  3.00  2.00 12.00 GGTCAGCCACACCAAAATCT
   PRODUCT SIZE: 595, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       2176   20   60.02   55.00  3.00  1.00 11.00 GGCGGCACTTTTCTCTACTG
   RIGHT PRIMER      2705   20   59.96   50.00  4.00  3.00 12.00 GCCAGGTTTCTATGCACCAT
   PRODUCT SIZE: 530, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00