PRIMER PICKING RESULTS FOR NM_002005,Homo sapiens feline sarcoma oncogene (FES), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        605   20   60.00   55.00  4.00  1.00 11.00 GGAAGCTCTTTGCTCACCAC
RIGHT PRIMER       865   20   60.01   55.00  6.00  3.00 11.00 AGCCTTGGTACTCAGCCTCA
SEQUENCE SIZE: 2762
INCLUDED REGION SIZE: 2762

PRODUCT SIZE: 261, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER        603   20   60.00   55.00  4.00  1.00 10.00 GTGGAAGCTCTTTGCTCACC
   RIGHT PRIMER       865   20   60.01   55.00  6.00  3.00 11.00 AGCCTTGGTACTCAGCCTCA
   PRODUCT SIZE: 263, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER        605   20   60.00   55.00  4.00  1.00 11.00 GGAAGCTCTTTGCTCACCAC
   RIGHT PRIMER       938   20   59.99   60.00  5.00  3.00 11.00 CCCTCCTCAAGCAGTGACTC
   PRODUCT SIZE: 334, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        603   20   60.00   55.00  4.00  1.00 10.00 GTGGAAGCTCTTTGCTCACC
   RIGHT PRIMER       938   20   59.99   60.00  5.00  3.00 11.00 CCCTCCTCAAGCAGTGACTC
   PRODUCT SIZE: 336, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER        605   20   60.00   55.00  4.00  1.00 11.00 GGAAGCTCTTTGCTCACCAC
   RIGHT PRIMER      1114   20   60.09   55.00  2.00  1.00 12.00 GGTGGGTGTTCTCCTCTTCA
   PRODUCT SIZE: 510, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00