PRIMER PICKING RESULTS FOR NM_002011,Homo sapiens fibroblast growth factor receptor 4 (FGFR4), transcript variant 1, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        286   20   60.01   60.00  5.00  2.00 12.00 GAGCTGACAGTAGCCCTTGG
RIGHT PRIMER       797   20   60.02   55.00  4.00  3.00 10.00 ACGCTCTCCATCACGAGACT
SEQUENCE SIZE: 3015
INCLUDED REGION SIZE: 3015

PRODUCT SIZE: 512, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER        286   20   60.01   60.00  5.00  2.00 12.00 GAGCTGACAGTAGCCCTTGG
   RIGHT PRIMER       610   20   60.03   60.00  2.00  1.00 11.00 GGTGTGTCCAGTAGGGTGCT
   PRODUCT SIZE: 325, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER        591   20   60.03   60.00  2.00  0.00 11.00 AGCACCCTACTGGACACACC
   RIGHT PRIMER       797   20   60.02   55.00  4.00  3.00 10.00 ACGCTCTCCATCACGAGACT
   PRODUCT SIZE: 207, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       1671   20   60.02   50.00  3.00  3.00 11.00 CAAAGACAACGCCTCTGACA
   RIGHT PRIMER      2658   20   59.97   50.00  4.00  2.00 11.00 CATGCTATCAAGGTCGAGCA
   PRODUCT SIZE: 988, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER        286   20   60.01   60.00  5.00  2.00 12.00 GAGCTGACAGTAGCCCTTGG
   RIGHT PRIMER       795   20   59.95   60.00  5.00  3.00 11.00 GCTCTCCATCACGAGACTCC
   PRODUCT SIZE: 510, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00