PRIMER PICKING RESULTS FOR NM_002019,Homo sapiens fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) (FLT1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2461   20   60.00   55.00  4.00  2.00 10.00 GGCTCTGTGGAAAGTTCAGC
RIGHT PRIMER      2683   20   59.99   50.00  3.00  0.00 10.00 GCTCACACTGCTCATCCAAA
SEQUENCE SIZE: 7680
INCLUDED REGION SIZE: 7680

PRODUCT SIZE: 223, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       2461   20   60.00   55.00  4.00  2.00 10.00 GGCTCTGTGGAAAGTTCAGC
   RIGHT PRIMER      3055   20   59.99   50.00  5.00  0.00 10.75 GTGCTGCATCCTTGTTGAGA
   PRODUCT SIZE: 595, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER       2178   20   60.01   50.00  6.00  2.00 12.00 AGGGGAAGAAATCCTCCAGA
   RIGHT PRIMER      2683   20   59.99   50.00  3.00  0.00 10.00 GCTCACACTGCTCATCCAAA
   PRODUCT SIZE: 506, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       2178   20   60.01   50.00  6.00  2.00 12.00 AGGGGAAGAAATCCTCCAGA
   RIGHT PRIMER      3055   20   59.99   50.00  5.00  0.00 10.75 GTGCTGCATCCTTGTTGAGA
   PRODUCT SIZE: 878, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       2664   20   59.99   50.00  3.00  2.00 11.00 TTTGGATGAGCAGTGTGAGC
   RIGHT PRIMER      3055   20   59.99   50.00  5.00  0.00 10.75 GTGCTGCATCCTTGTTGAGA
   PRODUCT SIZE: 392, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00