PRIMER PICKING RESULTS FOR NM_002020,Homo sapiens fms-related tyrosine kinase 4 (FLT4), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        233   20   59.99   60.00  2.00  1.00 10.00 GAGACAAGGACAGCGAGGAC
RIGHT PRIMER      1195   20   59.99   60.00  3.00  2.00 11.00 CCTCTGTCACCTCCTTGAGC
SEQUENCE SIZE: 4450
INCLUDED REGION SIZE: 4450

PRODUCT SIZE: 963, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER        233   20   59.99   60.00  2.00  1.00 10.00 GAGACAAGGACAGCGAGGAC
   RIGHT PRIMER      1196   20   59.99   60.00  3.00  3.00 12.00 GCCTCTGTCACCTCCTTGAG
   PRODUCT SIZE: 964, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1176   20   59.99   60.00  3.00  0.00 12.00 GCTCAAGGAGGTGACAGAGG
   RIGHT PRIMER      1553   20   60.01   55.00  3.00  2.00 12.00 ACAAACTCGGTCCAGGTGTC
   PRODUCT SIZE: 378, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       2170   20   59.98   55.00  3.00  0.00 11.00 CTGCTGGAGGAAAAGTCTGG
   RIGHT PRIMER      2757   20   60.01   55.00  4.00  3.00 11.00 GAGGTTGACCACGTTGAGGT
   PRODUCT SIZE: 588, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       1534   20   60.01   55.00  3.00  3.00 10.00 GACACCTGGACCGAGTTTGT
   RIGHT PRIMER      2189   20   59.98   55.00  3.00  3.00 12.00 CCAGACTTTTCCTCCAGCAG
   PRODUCT SIZE: 656, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00