PRIMER PICKING RESULTS FOR NM_002031,Homo sapiens fyn-related kinase (FRK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        294   20   59.98   50.00  4.00  2.00 10.00 TGCTGGACTGATCTGAAACG
RIGHT PRIMER       786   20   60.01   55.00  6.00  0.00 10.00 TGCCTGTAGGCTTCTGTCCT
SEQUENCE SIZE: 2863
INCLUDED REGION SIZE: 2863

PRODUCT SIZE: 493, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER        294   20   59.98   50.00  4.00  2.00 10.00 TGCTGGACTGATCTGAAACG
   RIGHT PRIMER       787   20   60.01   60.00  6.00  1.00 10.00 CTGCCTGTAGGCTTCTGTCC
   PRODUCT SIZE: 494, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER        637   20   60.02   50.00  3.00  1.00 12.00 CGAGCAGGTGACAAACTTCA
   RIGHT PRIMER       786   20   60.01   55.00  6.00  0.00 10.00 TGCCTGTAGGCTTCTGTCCT
   PRODUCT SIZE: 150, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER        637   20   60.02   50.00  3.00  1.00 12.00 CGAGCAGGTGACAAACTTCA
   RIGHT PRIMER       787   20   60.01   60.00  6.00  1.00 10.00 CTGCCTGTAGGCTTCTGTCC
   PRODUCT SIZE: 151, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       2144   20   60.02   55.00  6.00  3.00 11.00 CTGCAGGACAGTCAAGGTGA
   RIGHT PRIMER      2694   20   59.98   45.00  6.00  2.00 10.00 AACTCATCCGTTTTGCAACC
   PRODUCT SIZE: 551, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00