PRIMER PICKING RESULTS FOR NM_002037,Homo sapiens FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        580   20   60.00   45.00  4.00  1.00  9.00 ATGGGCTGTGTGCAATGTAA
RIGHT PRIMER      1578   20   60.01   55.00  4.00  2.00 11.00 CACCACTGCATAGAGCTGGA
SEQUENCE SIZE: 2650
INCLUDED REGION SIZE: 2650

PRODUCT SIZE: 999, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       1425   20   60.01   50.00  2.00  2.00 12.00 TGGGGAAGTATGGATGGGTA
   RIGHT PRIMER      1578   20   60.01   55.00  4.00  2.00 11.00 CACCACTGCATAGAGCTGGA
   PRODUCT SIZE: 154, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER        580   20   60.00   45.00  4.00  1.00  9.00 ATGGGCTGTGTGCAATGTAA
   RIGHT PRIMER       705   20   60.01   60.00  4.00  3.00 10.00 GAAGCTGGGGTAGTGCTGAG
   PRODUCT SIZE: 126, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER        578   20   60.00   45.00  4.00  1.00 10.00 TAATGGGCTGTGTGCAATGT
   RIGHT PRIMER       705   20   60.01   60.00  4.00  3.00 10.00 GAAGCTGGGGTAGTGCTGAG
   PRODUCT SIZE: 128, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER        578   20   60.00   45.00  4.00  1.00 10.00 TAATGGGCTGTGTGCAATGT
   RIGHT PRIMER      1130   20   60.01   50.00  5.00  1.00 10.00 GCACCTTTGGTGGTTTCACT
   PRODUCT SIZE: 553, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00