PRIMER PICKING RESULTS FOR NM_002110,Homo sapiens hemopoietic cell kinase (HCK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1471   20   60.01   55.00  7.00  2.00 10.00 ATCCCTTACCCAGGGATGTC
RIGHT PRIMER      1791   20   59.96   60.00  2.00  0.00 12.00 GTGGGTGTCTGGGAGTAGGA
SEQUENCE SIZE: 2015
INCLUDED REGION SIZE: 2015

PRODUCT SIZE: 321, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       1470   20   60.01   55.00  7.00  3.00 11.00 GATCCCTTACCCAGGGATGT
   RIGHT PRIMER      1791   20   59.96   60.00  2.00  0.00 12.00 GTGGGTGTCTGGGAGTAGGA
   PRODUCT SIZE: 322, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER        965   20   59.98   50.00  3.00  0.00 11.00 TGGCAGTGAAGACGATGAAG
   RIGHT PRIMER      1791   20   59.96   60.00  2.00  0.00 12.00 GTGGGTGTCTGGGAGTAGGA
   PRODUCT SIZE: 827, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       1796   20   60.00   55.00  4.00  0.00 12.00 GCTTCAGCCACAGTTTCCTC
   RIGHT PRIMER      1960   20   60.05   45.00  3.00  2.00 11.00 TTTCCCTTCCAAATCACAGC
   PRODUCT SIZE: 165, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       1508   20   59.98   60.00  6.00  2.00 11.00 GAGCTCTGGAGCGTGGATAC
   RIGHT PRIMER      1791   20   59.96   60.00  2.00  0.00 12.00 GTGGGTGTCTGGGAGTAGGA
   PRODUCT SIZE: 284, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00