PRIMER PICKING RESULTS FOR NM_002227,Homo sapiens Janus kinase 1 (a protein tyrosine kinase) (JAK1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2054   20   60.01   60.00  4.00  0.00 10.00 GGGGTCCTCTGGATCTCTTC
RIGHT PRIMER      2754   20   59.99   55.00  2.00  0.00 12.00 CACCTGCTCCCCTGTATTGT
SEQUENCE SIZE: 3541
INCLUDED REGION SIZE: 3541

PRODUCT SIZE: 701, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       2137   20   60.01   55.00  5.00  0.00 11.00 AGTGCCCTGAGCTACTTGGA
   RIGHT PRIMER      2754   20   59.99   55.00  2.00  0.00 12.00 CACCTGCTCCCCTGTATTGT
   PRODUCT SIZE: 618, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       2138   20   60.01   60.00  4.00  2.00 10.00 GTGCCCTGAGCTACTTGGAG
   RIGHT PRIMER      2754   20   59.99   55.00  2.00  0.00 12.00 CACCTGCTCCCCTGTATTGT
   PRODUCT SIZE: 617, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       2488   20   60.01   55.00  4.00  1.00 11.00 TGTAAGGAGCTGGCTGACCT
   RIGHT PRIMER      2754   20   59.99   55.00  2.00  0.00 12.00 CACCTGCTCCCCTGTATTGT
   PRODUCT SIZE: 267, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       2054   20   60.01   60.00  4.00  0.00 10.00 GGGGTCCTCTGGATCTCTTC
   RIGHT PRIMER      2507   20   60.01   55.00  4.00  2.00 11.00 AGGTCAGCCAGCTCCTTACA
   PRODUCT SIZE: 454, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00