PRIMER PICKING RESULTS FOR NM_002344,Homo sapiens leukocyte tyrosine kinase (LTK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1423   20   60.00   50.00  3.00  3.00  9.00 CAGCCCCCAATCCCTATTAT
RIGHT PRIMER      2257   20   59.99   55.00  6.00  0.00  9.00 GTCATGATGCGGTACACAGG
SEQUENCE SIZE: 2940
INCLUDED REGION SIZE: 2940

PRODUCT SIZE: 835, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       1423   20   60.00   50.00  3.00  3.00  9.00 CAGCCCCCAATCCCTATTAT
   RIGHT PRIMER      2258   20   59.99   55.00  6.00  2.00  9.00 GGTCATGATGCGGTACACAG
   PRODUCT SIZE: 836, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1299   20   59.99   55.00  4.00  0.00 11.00 AGCCTCCTTATGGTGTGTGG
   RIGHT PRIMER      2257   20   59.99   55.00  6.00  0.00  9.00 GTCATGATGCGGTACACAGG
   PRODUCT SIZE: 959, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1299   20   59.99   55.00  4.00  0.00 11.00 AGCCTCCTTATGGTGTGTGG
   RIGHT PRIMER      2258   20   59.99   55.00  6.00  2.00  9.00 GGTCATGATGCGGTACACAG
   PRODUCT SIZE: 960, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       1423   20   60.00   50.00  3.00  3.00  9.00 CAGCCCCCAATCCCTATTAT
   RIGHT PRIMER      2159   20   59.98   55.00  4.00  3.00 10.00 ATAGGGCATGTAGCCCAGTG
   PRODUCT SIZE: 737, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00