PRIMER PICKING RESULTS FOR NM_002350,Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1490   20   60.01   55.00  4.00  3.00 12.00 CAGCAAGGGAAGGTGCTAAG
RIGHT PRIMER      1750   20   59.99   50.00  3.00  0.00 11.00 CTGCCTTTTCTTTCCAGCAC
SEQUENCE SIZE: 2298
INCLUDED REGION SIZE: 2298

PRODUCT SIZE: 261, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       1490   20   60.01   55.00  4.00  3.00 12.00 CAGCAAGGGAAGGTGCTAAG
   RIGHT PRIMER      2146   20   59.97   45.00  4.00  0.00 11.00 AGTTGGGTGCATTTTTGGAG
   PRODUCT SIZE: 657, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1397   20   60.04   50.00  6.00  2.00  9.00 ACCTGCGAGCAGCTAATGTT
   RIGHT PRIMER      1750   20   59.99   50.00  3.00  0.00 11.00 CTGCCTTTTCTTTCCAGCAC
   PRODUCT SIZE: 354, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1286   20   59.95   50.00  3.00  0.00 12.00 TCCTGAAGAGCGATGAAGGT
   RIGHT PRIMER      1750   20   59.99   50.00  3.00  0.00 11.00 CTGCCTTTTCTTTCCAGCAC
   PRODUCT SIZE: 465, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       1490   20   60.01   55.00  4.00  3.00 12.00 CAGCAAGGGAAGGTGCTAAG
   RIGHT PRIMER      2142   20   60.05   45.00  4.00  0.00 11.00 GGGTGCATTTTTGGAGAGAA
   PRODUCT SIZE: 653, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00