PRIMER PICKING RESULTS FOR NM_002378,Homo sapiens megakaryocyte-associated tyrosine kinase (MATK), transcript variant 2, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        890   20   60.02   50.00  4.00  1.00 12.00 CGTGTTCTTCTGCAACCTCA
RIGHT PRIMER      1549   20   60.01   50.00  5.00  0.00 10.00 CTGGTGAACTTCCCGTGTTT
SEQUENCE SIZE: 2104
INCLUDED REGION SIZE: 2104

PRODUCT SIZE: 660, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER        890   20   60.02   50.00  4.00  1.00 12.00 CGTGTTCTTCTGCAACCTCA
   RIGHT PRIMER      1200   20   59.99   50.00  4.00  1.00 10.00 TCTCGTGTTGCATCTTCGTC
   PRODUCT SIZE: 311, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1057   20   59.94   60.00  4.00  0.00 12.00 CACAGATCGGAGAGGGAGAG
   RIGHT PRIMER      1549   20   60.01   50.00  5.00  0.00 10.00 CTGGTGAACTTCCCGTGTTT
   PRODUCT SIZE: 493, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1057   20   59.94   60.00  4.00  0.00 12.00 CACAGATCGGAGAGGGAGAG
   RIGHT PRIMER      1200   20   59.99   50.00  4.00  1.00 10.00 TCTCGTGTTGCATCTTCGTC
   PRODUCT SIZE: 144, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER        269   20   60.11   60.00  4.00  0.00 10.00 CTAGGCGACATCGAGAGAGG
   RIGHT PRIMER      1200   20   59.99   50.00  4.00  1.00 10.00 TCTCGTGTTGCATCTTCGTC
   PRODUCT SIZE: 932, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00