PRIMER PICKING RESULTS FOR NM_002529,Homo sapiens neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1134   20   59.97   50.00  4.00  1.00  9.00 CATGGACAACCCTTTCGAGT
RIGHT PRIMER      1512   20   60.01   55.00  5.00  3.00 11.00 GTGGTGAACACAGGCATCAC
SEQUENCE SIZE: 2391
INCLUDED REGION SIZE: 2391

PRODUCT SIZE: 379, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER        687   20   59.91   60.00  4.00  3.00 12.00 CACAGAGCTGGAGCAGTCAG
   RIGHT PRIMER      1512   20   60.01   55.00  5.00  3.00 11.00 GTGGTGAACACAGGCATCAC
   PRODUCT SIZE: 826, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1316   20   60.09   45.00  6.00  0.00 11.00 TCAACAAATGTGGACGGAGA
   RIGHT PRIMER      1512   20   60.01   55.00  5.00  3.00 11.00 GTGGTGAACACAGGCATCAC
   PRODUCT SIZE: 197, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       1137   20   60.09   50.00  4.00  1.00  9.00 GGACAACCCTTTCGAGTTCA
   RIGHT PRIMER      1512   20   60.01   55.00  5.00  3.00 11.00 GTGGTGAACACAGGCATCAC
   PRODUCT SIZE: 376, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       1136   20   60.09   50.00  4.00  2.00 10.00 TGGACAACCCTTTCGAGTTC
   RIGHT PRIMER      1512   20   60.01   55.00  5.00  3.00 11.00 GTGGTGAACACAGGCATCAC
   PRODUCT SIZE: 377, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00