PRIMER PICKING RESULTS FOR NM_002530,Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1702   20   60.01   55.00  5.00  1.00 10.00 ACTTCCGTCAGGGACACAAC
RIGHT PRIMER      1920   20   60.01   55.00  4.00  2.00 10.00 CCTCCCTCTGGAAATCCTTC
SEQUENCE SIZE: 2750
INCLUDED REGION SIZE: 2750

PRODUCT SIZE: 219, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       1702   20   60.01   55.00  5.00  1.00 10.00 ACTTCCGTCAGGGACACAAC
   RIGHT PRIMER      2526   20   59.99   55.00  5.00  0.00 10.00 CCAGCATGACATCGTACACC
   PRODUCT SIZE: 825, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1702   20   60.01   55.00  5.00  1.00 10.00 ACTTCCGTCAGGGACACAAC
   RIGHT PRIMER      2527   20   59.99   55.00  5.00  2.00 10.00 CCCAGCATGACATCGTACAC
   PRODUCT SIZE: 826, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       1702   20   60.01   55.00  5.00  1.00 10.00 ACTTCCGTCAGGGACACAAC
   RIGHT PRIMER      2232   20   59.98   55.00  5.00  2.00 10.00 CTAGCAGATTCGCTCCAACC
   PRODUCT SIZE: 531, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       1287   20   59.97   45.00  5.00  1.00 12.00 CTCATTGCCAAAAACCCACT
   RIGHT PRIMER      1920   20   60.01   55.00  4.00  2.00 10.00 CCTCCCTCTGGAAATCCTTC
   PRODUCT SIZE: 634, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00