PRIMER PICKING RESULTS FOR NM_002609,Homo sapiens platelet-derived growth factor receptor, beta polypeptide (PDGFRB), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       4689   20   60.01   50.00  4.00  2.00 10.00 CAGGTGGTTGCACATTTGTC
RIGHT PRIMER      5382   20   60.00   50.00  5.00  2.00  9.00 GTAAGGTGCCAACCTGCAAT
SEQUENCE SIZE: 5598
INCLUDED REGION SIZE: 5598

PRODUCT SIZE: 694, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       4305   20   60.01   55.00  7.00  1.00 12.00 AGGCTGAGCCAAGTACAGGA
   RIGHT PRIMER      4711   20   60.01   50.00  6.00  2.00 11.00 CTGGACAAATGTGCAACCAC
   PRODUCT SIZE: 407, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       4303   20   60.01   60.00  7.00  1.00 11.00 GGAGGCTGAGCCAAGTACAG
   RIGHT PRIMER      4711   20   60.01   50.00  6.00  2.00 11.00 CTGGACAAATGTGCAACCAC
   PRODUCT SIZE: 409, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       4305   20   60.01   55.00  7.00  1.00 12.00 AGGCTGAGCCAAGTACAGGA
   RIGHT PRIMER      4708   20   60.01   50.00  4.00  2.00  9.00 GACAAATGTGCAACCACCTG
   PRODUCT SIZE: 404, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER       4303   20   60.01   60.00  7.00  1.00 11.00 GGAGGCTGAGCCAAGTACAG
   RIGHT PRIMER      4708   20   60.01   50.00  4.00  2.00  9.00 GACAAATGTGCAACCACCTG
   PRODUCT SIZE: 406, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00