PRIMER PICKING RESULTS FOR NM_002821,Homo sapiens PTK7 protein tyrosine kinase 7 (PTK7), transcript variant 1, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        782   20   60.00   55.00  5.00  2.00 10.00 GCCAGAACTTCACCTTGAGC
RIGHT PRIMER      1376   20   60.01   55.00  8.00  3.00 12.00 GTGGCCACAGTGATGTTGAC
SEQUENCE SIZE: 4191
INCLUDED REGION SIZE: 4191

PRODUCT SIZE: 595, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER        576   20   60.01   55.00  6.00  1.00 10.00 GACCCAGGTCACACTTCGTT
   RIGHT PRIMER      1145   20   59.99   50.00  4.00  1.00 12.00 AACACCCGTGGCTCAAATAG
   PRODUCT SIZE: 570, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1126   20   59.99   50.00  4.00  0.00 11.00 CTATTTGAGCCACGGGTGTT
   RIGHT PRIMER      1376   20   60.01   55.00  8.00  3.00 12.00 GTGGCCACAGTGATGTTGAC
   PRODUCT SIZE: 251, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        782   20   60.00   55.00  5.00  2.00 10.00 GCCAGAACTTCACCTTGAGC
   RIGHT PRIMER      1409   20   59.98   55.00  3.00  2.00 11.00 CTGTCTTGGGGCTTCTTCAG
   PRODUCT SIZE: 628, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       1126   20   59.99   50.00  4.00  0.00 11.00 CTATTTGAGCCACGGGTGTT
   RIGHT PRIMER      1409   20   59.98   55.00  3.00  2.00 11.00 CTGTCTTGGGGCTTCTTCAG
   PRODUCT SIZE: 284, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00