PRIMER PICKING RESULTS FOR NM_003177,Homo sapiens spleen tyrosine kinase (SYK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1433   20   60.00   55.00  4.00  1.00 10.00 AGCTGGACAACCCGTACATC
RIGHT PRIMER      1885   20   60.00   50.00  4.00  0.00 11.00 TCATCCCTCGATATGGCTTC
SEQUENCE SIZE: 2681
INCLUDED REGION SIZE: 2681

PRODUCT SIZE: 453, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       1866   20   60.00   50.00  4.00  1.00 11.00 GAAGCCATATCGAGGGATGA
   RIGHT PRIMER      2204   20   60.01   45.00  7.00  0.00 10.00 TGACAAGTTGTGGGCATGTT
   PRODUCT SIZE: 339, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1866   20   60.00   50.00  4.00  1.00 11.00 GAAGCCATATCGAGGGATGA
   RIGHT PRIMER      1996   20   59.99   55.00  6.00  3.00 12.00 CCACATCGTATGTCCAGCAC
   PRODUCT SIZE: 131, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1433   20   60.00   55.00  4.00  1.00 10.00 AGCTGGACAACCCGTACATC
   RIGHT PRIMER      2204   20   60.01   45.00  7.00  0.00 10.00 TGACAAGTTGTGGGCATGTT
   PRODUCT SIZE: 772, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       1433   20   60.00   55.00  4.00  1.00 10.00 AGCTGGACAACCCGTACATC
   RIGHT PRIMER      1996   20   59.99   55.00  6.00  3.00 12.00 CCACATCGTATGTCCAGCAC
   PRODUCT SIZE: 564, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 2.00