PRIMER PICKING RESULTS FOR NM_003215,Homo sapiens tec protein tyrosine kinase (TEC), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER         83   20   59.99   55.00  3.00  1.00 11.00 TCTTCAGTGGCAGGAGGAGT
RIGHT PRIMER       701   20   59.96   50.00  3.00  2.00 12.00 CCTTCTGCTGCTTGGAAATC
SEQUENCE SIZE: 3650
INCLUDED REGION SIZE: 3650

PRODUCT SIZE: 619, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER        682   20   59.96   50.00  3.00  0.00 12.00 GATTTCCAAGCAGCAGAAGG
   RIGHT PRIMER      1126   20   60.02   50.00  5.00  2.00 10.00 GTCCTGCTGCATTGTGCTTA
   PRODUCT SIZE: 445, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER         83   20   59.99   55.00  3.00  1.00 11.00 TCTTCAGTGGCAGGAGGAGT
   RIGHT PRIMER       343   20   59.94   45.00  3.00  0.00 11.00 TTTGACAGGGAATGACACCA
   PRODUCT SIZE: 261, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       3174   20   59.99   55.00  3.00  2.00 11.00 AGGAGACCCTGCTGTCAAGA
   RIGHT PRIMER      3610   20   60.07   50.00  2.00  0.00 11.00 TATGAGGGGCACAGGAAAAG
   PRODUCT SIZE: 437, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER         83   20   59.99   55.00  3.00  1.00 11.00 TCTTCAGTGGCAGGAGGAGT
   RIGHT PRIMER       543   20   60.07   45.00  4.00  1.00 10.00 TCCGGGTGCTAATTTTTCAG
   PRODUCT SIZE: 461, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00