PRIMER PICKING RESULTS FOR NM_003328,Homo sapiens TXK tyrosine kinase (TXK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        744   20   60.01   45.00  3.00  1.00 11.00 GCCTTTCAATCAATCCCTGA
RIGHT PRIMER      1032   20   60.01   45.00  7.00  3.00 12.00 TGGCCTCTTCAATGAAATCC
SEQUENCE SIZE: 2564
INCLUDED REGION SIZE: 2564

PRODUCT SIZE: 289, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER        744   20   60.01   45.00  3.00  1.00 11.00 GCCTTTCAATCAATCCCTGA
   RIGHT PRIMER      1695   20   59.97   45.00  3.00  0.00 12.00 CTTTGGGTTGGCATTCTGTT
   PRODUCT SIZE: 952, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER        744   20   60.01   45.00  3.00  1.00 11.00 GCCTTTCAATCAATCCCTGA
   RIGHT PRIMER      1396   20   59.97   55.00  5.00  3.00 10.00 ACTTCAGGAGGGGACCACTT
   PRODUCT SIZE: 653, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        108   20   59.97   50.00  3.00  1.00 12.00 ACCATCCAGTCGGTTTTCTG
   RIGHT PRIMER      1032   20   60.01   45.00  7.00  3.00 12.00 TGGCCTCTTCAATGAAATCC
   PRODUCT SIZE: 925, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER        744   20   60.01   45.00  3.00  1.00 11.00 GCCTTTCAATCAATCCCTGA
   RIGHT PRIMER       848   20   59.96   45.00  3.00  1.00 11.00 TGGTAAACAACTGCCCATCA
   PRODUCT SIZE: 105, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00