PRIMER PICKING RESULTS FOR NM_003331,Homo sapiens tyrosine kinase 2 (TYK2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       3226   20   60.01   55.00  6.00  2.00 10.00 CTGCAGCTGGTCATGGAGTA
RIGHT PRIMER      3591   20   60.01   55.00  5.00  1.00 12.00 CAGCAGCTCATACAGGGTCA
SEQUENCE SIZE: 4176
INCLUDED REGION SIZE: 4176

PRODUCT SIZE: 366, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       3165   20   60.02   55.00  4.00  1.00 11.00 GCTCTACCACGAGCACATCA
   RIGHT PRIMER      3591   20   60.01   55.00  5.00  1.00 12.00 CAGCAGCTCATACAGGGTCA
   PRODUCT SIZE: 427, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       3226   20   60.01   55.00  6.00  2.00 10.00 CTGCAGCTGGTCATGGAGTA
   RIGHT PRIMER      3667   20   59.98   55.00  6.00  2.00 12.00 CTGTCATCTGACCCTGAGCA
   PRODUCT SIZE: 442, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER       3165   20   60.02   55.00  4.00  1.00 11.00 GCTCTACCACGAGCACATCA
   RIGHT PRIMER      3667   20   59.98   55.00  6.00  2.00 12.00 CTGTCATCTGACCCTGAGCA
   PRODUCT SIZE: 503, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       1709   20   60.00   55.00  4.00  0.00 10.00 ACGGCCTGTACCTCATTCAC
   RIGHT PRIMER      2078   20   60.05   55.00  6.00  1.00 10.00 TGGGTGATCTCCTTCTGGTC
   PRODUCT SIZE: 370, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00