PRIMER PICKING RESULTS FOR NM_003985,Homo sapiens tyrosine kinase, non-receptor, 1 (TNK1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        133   20   60.01   60.00  4.00  3.00 10.00 GCTCCCTGTGGCTACTGAAG
RIGHT PRIMER       613   20   59.97   55.00  4.00  2.00 10.00 AGGAAGTCCCCCAGTTCTGT
SEQUENCE SIZE: 2771
INCLUDED REGION SIZE: 2771

PRODUCT SIZE: 481, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       1680   20   59.96   50.00  5.00  2.00 12.00 GAAATTCGACAAGCCAGAGC
   RIGHT PRIMER      1924   20   59.99   50.00  4.00  0.00 11.00 TGCACACTCAGCTCCATTTC
   PRODUCT SIZE: 245, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER        166   20   59.97   50.00  5.00  1.00 10.00 TCCAGTTGGCCCAGTTTTAC
   RIGHT PRIMER       613   20   59.97   55.00  4.00  2.00 10.00 AGGAAGTCCCCCAGTTCTGT
   PRODUCT SIZE: 448, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER        624   20   60.05   45.00  7.00  1.00 12.00 TCGGTCATGATGAACTTGGA
   RIGHT PRIMER      1617   20   60.01   45.00  8.00  0.00 11.00 TGGAAATGCCTTTCATCCTC
   PRODUCT SIZE: 994, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER        624   20   60.05   45.00  7.00  1.00 12.00 TCGGTCATGATGAACTTGGA
   RIGHT PRIMER      1620   20   60.01   45.00  8.00  2.00 10.00 TCCTGGAAATGCCTTTCATC
   PRODUCT SIZE: 997, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00