PRIMER PICKING RESULTS FOR NM_004103,Homo sapiens protein tyrosine kinase 2 beta (PTK2B), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2121   20   59.98   55.00  4.00  0.00 12.00 CTTCTGGCTGGAGAACAAGG
RIGHT PRIMER      2978   20   60.00   50.00  4.00  2.00 11.00 TTCTTCACCACCACCACGTA
SEQUENCE SIZE: 4151
INCLUDED REGION SIZE: 4151

PRODUCT SIZE: 858, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       1783   20   59.99   55.00  3.00  2.00 11.00 CTGGAGCGGAACAAGAACTC
   RIGHT PRIMER      2140   20   59.98   55.00  4.00  3.00 12.00 CCTTGTTCTCCAGCCAGAAG
   PRODUCT SIZE: 358, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1651   20   60.02   50.00  6.00  1.00 10.00 AAGTTCATGAGCGAGGCAGT
   RIGHT PRIMER      1802   20   59.99   55.00  3.00  2.00 11.00 GAGTTCTTGTTCCGCTCCAG
   PRODUCT SIZE: 152, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1651   20   60.02   50.00  6.00  1.00 10.00 AAGTTCATGAGCGAGGCAGT
   RIGHT PRIMER      2140   20   59.98   55.00  4.00  3.00 12.00 CCTTGTTCTCCAGCCAGAAG
   PRODUCT SIZE: 490, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       1783   20   59.99   55.00  3.00  2.00 11.00 CTGGAGCGGAACAAGAACTC
   RIGHT PRIMER      1963   20   59.96   50.00  4.00  0.00 12.00 TGTACCGGGAAAGACCAAAG
   PRODUCT SIZE: 181, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00