PRIMER PICKING RESULTS FOR NM_004119,Homo sapiens fms-related tyrosine kinase 3 (FLT3), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        734   20   60.00   55.00  3.00  3.00  9.00 GGACGGACATAAGGTGCTGT
RIGHT PRIMER      1454   20   60.04   50.00  4.00  3.00 12.00 TTCTTCCAGGTCCAAGATGG
SEQUENCE SIZE: 3475
INCLUDED REGION SIZE: 3475

PRODUCT SIZE: 721, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER        734   20   60.00   55.00  3.00  3.00  9.00 GGACGGACATAAGGTGCTGT
   RIGHT PRIMER      1627   20   59.93   45.00  4.00  2.00 11.00 AAGATGTGCCAAGGGAATTG
   PRODUCT SIZE: 894, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1435   20   60.04   50.00  4.00  2.00 12.00 CCATCTTGGACCTGGAAGAA
   RIGHT PRIMER      1925   20   60.05   45.00  4.00  2.00 11.00 TTTCCAAAAGCACCTGATCC
   PRODUCT SIZE: 491, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        734   20   60.00   55.00  3.00  3.00  9.00 GGACGGACATAAGGTGCTGT
   RIGHT PRIMER      1298   20   60.10   50.00  3.00  1.00 10.00 CCTGGCTGGTGCTTATGATT
   PRODUCT SIZE: 565, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       2518   20   59.97   50.00  3.00  2.00 11.00 CACGGGAAAGTGGTGAAGAT
   RIGHT PRIMER      3077   20   59.93   55.00  3.00  2.00 11.00 AGGTGGAGGGATGAAGTCCT
   PRODUCT SIZE: 560, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00