PRIMER PICKING RESULTS FOR NM_004304,Homo sapiens anaplastic lymphoma kinase (Ki-1) (ALK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1703   20   60.00   55.00  4.00  2.00 10.00 GGAGTGCAGCTTTGACTTCC
RIGHT PRIMER      2412   20   60.01   60.00  3.00  3.00  9.00 ACTGAGGAGTGTGGGGTGAC
SEQUENCE SIZE: 6989
INCLUDED REGION SIZE: 6989

PRODUCT SIZE: 710, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       1703   20   60.00   55.00  4.00  2.00 10.00 GGAGTGCAGCTTTGACTTCC
   RIGHT PRIMER      2409   20   60.01   60.00  3.00  3.00 12.00 GAGGAGTGTGGGGTGACAGT
   PRODUCT SIZE: 707, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER       4665   20   60.00   55.00  3.00  1.00 11.00 GCCAGAAACTGCCTCTTGAC
   RIGHT PRIMER      5028   20   59.99   50.00  7.00  2.00 12.00 TGTCTTCAGGCTGATGTTGC
   PRODUCT SIZE: 364, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER       4665   20   60.00   55.00  3.00  1.00 11.00 GCCAGAAACTGCCTCTTGAC
   RIGHT PRIMER      5499   20   59.99   50.00  2.00  0.00 12.00 TCTCGTGTGGCTCCTTCTTT
   PRODUCT SIZE: 835, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       1093   20   60.01   60.00  2.00  2.00 10.00 CCTCGCTCTTCCGTGTCTAC
   RIGHT PRIMER      1722   20   60.00   55.00  4.00  2.00 11.00 GGAAGTCAAAGCTGCACTCC
   PRODUCT SIZE: 630, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00