PRIMER PICKING RESULTS FOR NM_004383,Homo sapiens c-src tyrosine kinase (CSK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        803   20   59.96   45.00  6.00  2.00 11.00 ATCATGTACCATGCCAGCAA
RIGHT PRIMER      1258   20   60.02   60.00  4.00  1.00 10.00 ACCCCTAGACCGCAGGTAGT
SEQUENCE SIZE: 2420
INCLUDED REGION SIZE: 2420

PRODUCT SIZE: 456, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER        803   20   59.96   45.00  6.00  2.00 11.00 ATCATGTACCATGCCAGCAA
   RIGHT PRIMER      1533   20   59.97   50.00  5.00  2.00 10.00 ATTCCGAAACTCCACACGTC
   PRODUCT SIZE: 731, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1514   20   59.97   50.00  5.00  3.00  9.00 GACGTGTGGAGTTTCGGAAT
   RIGHT PRIMER      1731   20   60.06   60.00  6.00  3.00 10.00 AGCTGCTCTCGGAGCTGTAG
   PRODUCT SIZE: 218, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       1319   20   59.93   50.00  4.00  0.00 12.00 GAATACCTGGAGGGCAACAA
   RIGHT PRIMER      1533   20   59.97   50.00  5.00  2.00 10.00 ATTCCGAAACTCCACACGTC
   PRODUCT SIZE: 215, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER        803   20   59.96   45.00  6.00  2.00 11.00 ATCATGTACCATGCCAGCAA
   RIGHT PRIMER      1731   20   60.06   60.00  6.00  3.00 10.00 AGCTGCTCTCGGAGCTGTAG
   PRODUCT SIZE: 929, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00