PRIMER PICKING RESULTS FOR NM_004431,Homo sapiens EphA2 (EPHA2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2058   20   60.02   50.00  8.00  1.00 12.00 AAAGCCGGCTACACAGAGAA
RIGHT PRIMER      2383   20   60.02   55.00  4.00  1.00  9.00 TCAGACACCTTGCAGACCAG
SEQUENCE SIZE: 3921
INCLUDED REGION SIZE: 3921

PRODUCT SIZE: 326, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       2058   20   60.02   50.00  8.00  1.00 12.00 AAAGCCGGCTACACAGAGAA
   RIGHT PRIMER      2947   20   59.97   45.00  3.00  2.00 10.00 TTGATGTCGTCGTTGGTCAT
   PRODUCT SIZE: 890, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1976   20   59.97   55.00  1.00  0.00 12.00 AGGAGAGTTTGGGGAGGTGT
   RIGHT PRIMER      2383   20   60.02   55.00  4.00  1.00  9.00 TCAGACACCTTGCAGACCAG
   PRODUCT SIZE: 408, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       2121   20   59.97   45.00  3.00  3.00 11.00 TTCAGCCACCACAACATCAT
   RIGHT PRIMER      2383   20   60.02   55.00  4.00  1.00  9.00 TCAGACACCTTGCAGACCAG
   PRODUCT SIZE: 263, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       2364   20   60.02   55.00  4.00  3.00 10.00 CTGGTCTGCAAGGTGTCTGA
   RIGHT PRIMER      2947   20   59.97   45.00  3.00  2.00 10.00 TTGATGTCGTCGTTGGTCAT
   PRODUCT SIZE: 584, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00