PRIMER PICKING RESULTS FOR NM_004438,Homo sapiens EphA4 (EPHA4), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        109   20   59.96   50.00  4.00  3.00  9.00 TACCCCGCGAATGAAGTTAC
RIGHT PRIMER       445   20   59.99   45.00  3.00  2.00 10.00 TGATGAAACGCTCTTTGTCG
SEQUENCE SIZE: 3107
INCLUDED REGION SIZE: 3107

PRODUCT SIZE: 337, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       1329   20   60.04   55.00  5.00  0.00 10.00 CACTGTGACCACCAACCAAG
   RIGHT PRIMER      2048   20   59.99   60.00  5.00  3.00 11.00 CTGGCCTCACTCAGGAAGTC
   PRODUCT SIZE: 720, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1329   20   60.04   55.00  5.00  0.00 10.00 CACTGTGACCACCAACCAAG
   RIGHT PRIMER      2047   20   59.99   55.00  5.00  3.00 10.00 TGGCCTCACTCAGGAAGTCT
   PRODUCT SIZE: 719, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER        308   20   59.96   55.00  5.00  2.00 11.00 AAGGGGCTCAGAGGGTGTAT
   RIGHT PRIMER       445   20   59.99   45.00  3.00  2.00 10.00 TGATGAAACGCTCTTTGTCG
   PRODUCT SIZE: 138, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER        522   20   59.98   50.00  4.00  0.00 11.00 CATGAAGCTGAACACCGAGA
   RIGHT PRIMER       704   20   59.96   55.00  4.00  2.00 10.00 GACGTATCAGCCCCTGTGAT
   PRODUCT SIZE: 183, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00