PRIMER PICKING RESULTS FOR NM_004440,Homo sapiens EphA7 (EPHA7), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER         81   20   60.01   50.00  4.00  1.00 11.00 CGATGCAAGGAGCACACTAA
RIGHT PRIMER      1000   20   60.02   45.00  4.00  0.00 11.00 TTTTGCTGGTAGCCTGCTTT
SEQUENCE SIZE: 4523
INCLUDED REGION SIZE: 4523

PRODUCT SIZE: 920, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER         81   20   60.01   50.00  4.00  1.00 11.00 CGATGCAAGGAGCACACTAA
   RIGHT PRIMER      1001   20   60.02   45.00  4.00  2.00 10.00 TTTTTGCTGGTAGCCTGCTT
   PRODUCT SIZE: 921, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER         81   20   60.01   50.00  4.00  1.00 11.00 CGATGCAAGGAGCACACTAA
   RIGHT PRIMER       889   20   59.97   55.00  6.00  1.00 11.00 ACACATGTCCCTCGAACCTC
   PRODUCT SIZE: 809, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER         81   20   60.01   50.00  4.00  1.00 11.00 CGATGCAAGGAGCACACTAA
   RIGHT PRIMER       890   20   59.97   55.00  6.00  1.00 10.00 GACACATGTCCCTCGAACCT
   PRODUCT SIZE: 810, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER        982   20   60.02   45.00  4.00  0.00 11.00 AAGCAGGCTACCAGCAAAAA
   RIGHT PRIMER      1911   20   59.97   50.00  4.00  0.00 11.00 AGCCAAAGACCATGAACACC
   PRODUCT SIZE: 930, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00