PRIMER PICKING RESULTS FOR NM_004441,Homo sapiens EphB1 (EPHB1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2178   20   60.01   60.00  5.00  1.00 12.00 CAGGGTACTCGGAGAAGCAG
RIGHT PRIMER      2790   20   60.01   55.00  7.00  2.00 12.00 TCCAGCATGAGCTGGTGTAG
SEQUENCE SIZE: 3865
INCLUDED REGION SIZE: 3865

PRODUCT SIZE: 613, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       1764   20   59.99   50.00  3.00  2.00 12.00 TCAGTGGCAAGATGTGCTTC
   RIGHT PRIMER      1957   20   60.01   50.00  4.00  0.00  9.00 ATGCTGGAGCTTATCGCTGT
   PRODUCT SIZE: 194, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER        281   20   60.00   55.00  3.00  0.00  9.00 ATGGACACCAGAACGGCTAC
   RIGHT PRIMER       608   20   59.99   60.00  4.00  2.00 11.00 CCTCAGACCAGAAGGCTGAC
   PRODUCT SIZE: 328, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       2178   20   60.01   60.00  5.00  1.00 12.00 CAGGGTACTCGGAGAAGCAG
   RIGHT PRIMER      2593   20   59.98   55.00  6.00  2.00 11.00 TGGAGCTGTCCATCTCACAG
   PRODUCT SIZE: 416, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER       1764   20   59.99   50.00  3.00  2.00 12.00 TCAGTGGCAAGATGTGCTTC
   RIGHT PRIMER      2593   20   59.98   55.00  6.00  2.00 11.00 TGGAGCTGTCCATCTCACAG
   PRODUCT SIZE: 830, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00