PRIMER PICKING RESULTS FOR NM_004443,Homo sapiens EphB3 (EPHB3), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        899   20   60.00   50.00  4.00  3.00 10.00 CGTGAAAGTGGACACCATTG
RIGHT PRIMER      1579   20   59.99   50.00  4.00  2.00 12.00 TGGCACTTCTTGCAGATGAC
SEQUENCE SIZE: 4234
INCLUDED REGION SIZE: 4234

PRODUCT SIZE: 681, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER        899   20   60.00   50.00  4.00  3.00 10.00 CGTGAAAGTGGACACCATTG
   RIGHT PRIMER      1578   20   59.99   50.00  4.00  3.00 12.00 GGCACTTCTTGCAGATGACA
   PRODUCT SIZE: 680, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER        289   20   60.04   50.00  4.00  0.00 11.00 ATTGCATTCCCTCCTCTCCT
   RIGHT PRIMER       918   20   60.00   50.00  4.00  2.00 10.00 CAATGGTGTCCACTTTCACG
   PRODUCT SIZE: 630, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        551   20   60.05   55.00  4.00  2.00 11.00 GGAAGAGACCCTCATGGACA
   RIGHT PRIMER       918   20   60.00   50.00  4.00  2.00 10.00 CAATGGTGTCCACTTTCACG
   PRODUCT SIZE: 368, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER        550   20   60.05   55.00  4.00  3.00 11.00 TGGAAGAGACCCTCATGGAC
   RIGHT PRIMER       918   20   60.00   50.00  4.00  2.00 10.00 CAATGGTGTCCACTTTCACG
   PRODUCT SIZE: 369, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00