PRIMER PICKING RESULTS FOR NM_004444,Homo sapiens EphB4 (EPHB4), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1805   20   59.96   45.00  3.00  0.00 11.00 TATTCGGACAAACACGGACA
RIGHT PRIMER      2027   20   59.99   50.00  4.00  2.00 10.00 TCTTGATTGCCACACAGCTC
SEQUENCE SIZE: 3945
INCLUDED REGION SIZE: 3945

PRODUCT SIZE: 223, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 1 LEFT PRIMER       1805   20   59.96   45.00  3.00  0.00 11.00 TATTCGGACAAACACGGACA
   RIGHT PRIMER      2025   20   59.99   50.00  4.00  2.00 10.00 TTGATTGCCACACAGCTCTC
   PRODUCT SIZE: 221, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER       2006   20   59.99   50.00  4.00  1.00 10.00 GAGAGCTGTGTGGCAATCAA
   RIGHT PRIMER      2112   20   59.96   50.00  4.00  1.00 11.00 ATATTGGGGTGCTCGAACTG
   PRODUCT SIZE: 107, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       2008   20   59.99   50.00  4.00  2.00 10.00 GAGCTGTGTGGCAATCAAGA
   RIGHT PRIMER      2112   20   59.96   50.00  4.00  1.00 11.00 ATATTGGGGTGCTCGAACTG
   PRODUCT SIZE: 105, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       2008   20   59.99   50.00  4.00  2.00 10.00 GAGCTGTGTGGCAATCAAGA
   RIGHT PRIMER      2352   20   60.06   50.00  4.00  1.00 10.00 ACTTTGCAGACGAGGTTGCT
   PRODUCT SIZE: 345, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00