PRIMER PICKING RESULTS FOR NM_004445,Homo sapiens EphB6 (EPHB6), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1722   20   59.96   55.00  3.00  2.00 10.00 GTCTTCTGCTGGGAATGCTC
RIGHT PRIMER      2435   20   59.96   50.00  3.00  2.00 11.00 CGCACCTGGAAACCATAGAT
SEQUENCE SIZE: 4010
INCLUDED REGION SIZE: 4010

PRODUCT SIZE: 714, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       1220   20   60.06   50.00  6.00  1.00 11.00 ACACAATTGCAGCAGACGAG
   RIGHT PRIMER      1741   20   59.96   55.00  3.00  1.00 10.00 GAGCATTCCCAGCAGAAGAC
   PRODUCT SIZE: 522, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       3248   20   60.08   40.00  6.00  2.00 10.00 TCATTGCACATGGAAAGCAT
   RIGHT PRIMER      3389   20   59.98   55.00  4.00  2.00 11.00 CGGAACTCCTGCTCTATTGC
   PRODUCT SIZE: 142, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       3248   20   60.08   40.00  6.00  2.00 10.00 TCATTGCACATGGAAAGCAT
   RIGHT PRIMER      3524   20   60.07   50.00  4.00  3.00 10.00 GTATCTGGCTTGCGGATCAT
   PRODUCT SIZE: 277, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       2641   20   59.92   55.00  8.00  2.00 11.00 GAGCAGCTGCAGCAATACAG
   RIGHT PRIMER      3524   20   60.07   50.00  4.00  3.00 10.00 GTATCTGGCTTGCGGATCAT
   PRODUCT SIZE: 884, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00