PRIMER PICKING RESULTS FOR NM_004448,Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1557   20   59.99   55.00  2.00  0.00 12.00 CCATAACACCCACCTCTGCT
RIGHT PRIMER      2119   20   59.98   55.00  6.00  3.00 12.00 CAGAGACGATGGACGTCAGA
SEQUENCE SIZE: 4530
INCLUDED REGION SIZE: 4530

PRODUCT SIZE: 563, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       3872   20   60.01   60.00  3.00  1.00 12.00 CTACGGCAGAGAACCCAGAG
   RIGHT PRIMER      4357   20   59.97   50.00  7.00  0.00 10.75 ACACCATTGCTGTTCCTTCC
   PRODUCT SIZE: 486, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER       2100   20   59.98   55.00  6.00  2.00 11.00 TCTGACGTCCATCGTCTCTG
   RIGHT PRIMER      2930   20   60.02   50.00  8.00  1.00 10.00 GGGATCCCATCGTAAGGTTT
   PRODUCT SIZE: 831, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       3890   20   60.03   60.00  4.00  2.00 10.00 AGTACCTGGGTCTGGACGTG
   RIGHT PRIMER      4083   20   59.98   55.00  3.00  0.00 11.00 CTGGGAACTCAAGCAGGAAG
   PRODUCT SIZE: 194, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       3890   20   60.03   60.00  4.00  2.00 10.00 AGTACCTGGGTCTGGACGTG
   RIGHT PRIMER      4357   20   59.97   50.00  7.00  0.00 10.75 ACACCATTGCTGTTCCTTCC
   PRODUCT SIZE: 468, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00