PRIMER PICKING RESULTS FOR NM_004560,Homo sapiens receptor tyrosine kinase-like orphan receptor 2 (ROR2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2446   20   59.97   50.00  4.00  0.00 11.00 GGGCAACCTTTCCAACTACA
RIGHT PRIMER      3338   20   60.02   50.00  4.00  1.00 10.00 GCATTTGCTGCTCACTACCA
SEQUENCE SIZE: 4091
INCLUDED REGION SIZE: 4091

PRODUCT SIZE: 893, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       2446   20   59.97   50.00  4.00  0.00 11.00 GGGCAACCTTTCCAACTACA
   RIGHT PRIMER      3342   20   60.02   45.00  6.00  1.00 11.00 AAAGGCATTTGCTGCTCACT
   PRODUCT SIZE: 897, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       2939   20   59.95   60.00  3.00  0.00 12.00 GAGGAGGAGGAAGGCTCTGT
   RIGHT PRIMER      3338   20   60.02   50.00  4.00  1.00 10.00 GCATTTGCTGCTCACTACCA
   PRODUCT SIZE: 400, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       2940   20   59.95   60.00  3.00  2.00 12.00 AGGAGGAGGAAGGCTCTGTC
   RIGHT PRIMER      3338   20   60.02   50.00  4.00  1.00 10.00 GCATTTGCTGCTCACTACCA
   PRODUCT SIZE: 399, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       2530   20   60.06   50.00  3.00  2.00 11.00 AGTGAGCAATGTGAGCAACG
   RIGHT PRIMER      3338   20   60.02   50.00  4.00  1.00 10.00 GCATTTGCTGCTCACTACCA
   PRODUCT SIZE: 809, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 3.00