PRIMER PICKING RESULTS FOR NM_004972,Homo sapiens Janus kinase 2 (a protein tyrosine kinase) (JAK2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        883   20   60.02   50.00  6.00  2.00 10.00 GAGCCTATCGGCATGGAATA
RIGHT PRIMER      1042   20   59.93   50.00  3.00  2.00 12.00 ACTGCCATCCCAAGACATTC
SEQUENCE SIZE: 5097
INCLUDED REGION SIZE: 5097

PRODUCT SIZE: 160, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER        883   20   60.02   50.00  6.00  2.00 10.00 GAGCCTATCGGCATGGAATA
   RIGHT PRIMER       993   20   59.93   40.00  6.00  2.00  9.00 TTATCCATCCGTGCACAAAA
   PRODUCT SIZE: 111, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER        883   20   60.02   50.00  6.00  2.00 10.00 GAGCCTATCGGCATGGAATA
   RIGHT PRIMER       995   20   59.93   40.00  6.00  3.00 11.00 TTTTATCCATCCGTGCACAA
   PRODUCT SIZE: 113, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER        883   20   60.02   50.00  6.00  2.00 10.00 GAGCCTATCGGCATGGAATA
   RIGHT PRIMER      1339   20   60.09   55.00  4.00  2.00 11.00 TCACCTGAAGGACCACTTCC
   PRODUCT SIZE: 457, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER        883   20   60.02   50.00  6.00  2.00 10.00 GAGCCTATCGGCATGGAATA
   RIGHT PRIMER      1504   20   60.09   45.00  4.00  2.00 10.00 TTTGAACCCTCTTGGTTTGC
   PRODUCT SIZE: 622, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00