PRIMER PICKING RESULTS FOR NM_005012,Homo sapiens receptor tyrosine kinase-like orphan receptor 1 (ROR1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1173   20   60.01   40.00  4.00  2.00 11.00 TGCAAGATCAAATCCCATGA
RIGHT PRIMER      1661   20   59.99   45.00  3.00  0.00 11.00 CGACAGACGCAAATGAAGAA
SEQUENCE SIZE: 3358
INCLUDED REGION SIZE: 3358

PRODUCT SIZE: 489, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       1173   20   60.01   40.00  4.00  2.00 11.00 TGCAAGATCAAATCCCATGA
   RIGHT PRIMER      1491   20   60.02   50.00  6.00  1.00 11.00 GGGAGCTTCCTTTTGATTCC
   PRODUCT SIZE: 319, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       2544   20   59.98   55.00  6.00  1.00 12.00 CCTCATGACAGAGTGCTGGA
   RIGHT PRIMER      2686   20   60.01   45.00  3.00  0.00 11.00 TTGTCTGTGTGGTGGCATTT
   PRODUCT SIZE: 143, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1642   20   59.99   45.00  3.00  3.00 11.00 TTCTTCATTTGCGTCTGTCG
   RIGHT PRIMER      2563   20   59.98   55.00  6.00  3.00 12.00 TCCAGCACTCTGTCATGAGG
   PRODUCT SIZE: 922, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER        927   20   59.98   55.00  6.00  2.00 11.00 CCGCACCGTCTATATGGAGT
   RIGHT PRIMER      1661   20   59.99   45.00  3.00  0.00 11.00 CGACAGACGCAAATGAAGAA
   PRODUCT SIZE: 735, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00